Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F1 I2 R1	67	32.5	1435938	95.9%	1377064	112.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,359	T→C	I221T (ATC→ACC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,359	0	T	C	100.0%	126.2 / NA	34	I221T (ATC→ACC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/20); total (14/20)

ATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGA  >  NC_000913/1756232‑1756484

aTCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAgg                                                                                                                    <  1:51732/139‑1 (MQ=255)

    ttGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAgg                                                                                                                >  2:358259/1‑139 (MQ=255)

           cGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTcaac                                                                                                         >  1:322300/1‑139 (MQ=255)

             aaCAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTcaacaa                                                                                                       >  1:636705/1‑139 (MQ=255)

                                     ctTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCctc                                                                               >  1:104568/1‑139 (MQ=255)

                                     ctTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCctc                                                                               <  2:636705/139‑1 (MQ=255)

                                        ccTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGa                                                                            <  1:358259/139‑1 (MQ=255)

                                                    aGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGt                                                                >  1:123971/1‑139 (MQ=255)

                                                        ttCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTgcg                                                            >  2:715852/1‑139 (MQ=255)

                                                            gACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTg                                                        <  2:104568/139‑1 (MQ=255)

                                                                ttATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGa                                                    >  1:493080/1‑139 (MQ=255)

                                                                 tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGATGCGCGTGGCGAc                                                   <  2:388335/139‑1 (MQ=255)

                                                                  aTCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGAcc                                                  <  2:123971/139‑1 (MQ=255)

                                                                  aTCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGATTGCGCGTGGCGAcc                                                  >  1:65213/1‑139 (MQ=255)

                                                                   tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         <  1:114915/115‑1 (MQ=255)

                                                                   tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         >  2:114915/1‑115 (MQ=255)

                                                                   tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCt                                                 <  1:125485/139‑1 (MQ=255)

                                                                   tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCt                                                 <  2:493080/139‑1 (MQ=255)

                                                                      aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTggg                                              <  1:715852/139‑1 (MQ=255)

                                                                      aaaTCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTggg                                              <  1:30196/139‑1 (MQ=255)

                                                                                gCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGATTGCGCGTGGCGACCTGGGTGTAGAAATc                                    <  2:65213/139‑1 (MQ=255)

                                                                                     tGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGt                               >  1:536040/1‑139 (MQ=255)

                                                                                                    gcgAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCg                                                                                      >  1:422786/1‑69 (MQ=255)

                                                                                                    gcgAAAACATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCg                                                                                      <  2:422786/69‑1 (MQ=255)

                                                                                                        aaaCATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTTTCTTCGcc            <  1:464805/139‑1 (MQ=255)

                                                                                                           cATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCag         <  2:251319/139‑1 (MQ=255)

                                                                                                           cATCCACATCATCTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCag         <  2:536040/139‑1 (MQ=255)

                                                                                                                  atcatcTCCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGTGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga  <  2:322300/139‑1 (MQ=255)

                                                                                                                      tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGAc                                                                           <  1:616990/62‑1 (MQ=255)

                                                                                                                      tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGAc                                                                           >  2:616990/1‑62 (MQ=255)

                                                                                                                      tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         >  2:700041/1‑64 (MQ=255)

                                                                                                                      tctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACgg                                                                         <  1:700041/64‑1 (MQ=255)

                                                                                                                       ctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAAt                                     >  1:651227/1‑99 (MQ=255)

                                                                                                                       ctcCAAAACCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAAt                                     <  2:651227/99‑1 (MQ=255)

ATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGA  >  NC_000913/1756232‑1756484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A