Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	67	31.4	1402038	96.1%	1347358	109.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,374	G→A	G226D (GGC→GAC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,374	0	G	A	100.0%	109.3 / NA	33	G226D (GGC→GAC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/16); total (17/16)

GTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAT  >  NC_000913/1756239‑1756500

gTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCt                                                                                                                             >  2:370003/1‑139 (MQ=255)

    cGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTcaac                                                                                                                         >  2:677933/1‑139 (MQ=255)

                     ttgttgCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCt                                                                                                        >  1:371221/1‑139 (MQ=255)

                                           tAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATg                                                                                  >  2:393934/1‑139 (MQ=255)

                                            aaGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATgg                                                                                 >  2:44609/1‑139 (MQ=255)

                                             aGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGt                                                                                >  2:5709/1‑139 (MQ=255)

                                                 ttCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTgcg                                                                            >  2:102885/1‑139 (MQ=255)

                                                 ttCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTgcg                                                                            >  1:574617/1‑139 (MQ=255)

                                                         ttATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGa                                                                    <  2:574617/139‑1 (MQ=255)

                                                          tATCGAAATCCGTGAGCACTTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGAc                                                                   <  2:329412/139‑1 (MQ=255)

                                                          tATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGAc                                                                   <  1:370003/139‑1 (MQ=255)

                                                            tCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCt                                                                 >  1:472895/1‑139 (MQ=255)

                                                                     gTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGa                                                        <  2:472895/139‑1 (MQ=255)

                                                                      tGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTgg                                                               >  1:102376/1‑131 (MQ=255)

                                                                      tGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTgg                                                               <  2:102376/131‑1 (MQ=255)

                                                                        aGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAAt                                                     <  2:627793/139‑1 (MQ=255)

                                                                           aCCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATccc                                                  <  1:102885/139‑1 (MQ=255)

                                                                             cTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCgg                                                >  2:132051/1‑139 (MQ=255)

                                                                                       aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTcgacg                                                                                                               >  1:597390/1‑66 (MQ=255)

                                                                                       aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTcgacg                                                                                                               <  2:597390/66‑1 (MQ=255)

                                                                                       aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCg                                                                                                      >  2:28184/1‑75 (MQ=255)

                                                                                       aCGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCg                                                                                                      <  1:28184/75‑1 (MQ=255)

                                                                                           cggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATc                                  >  1:118905/1‑139 (MQ=255)

                                                                                            ggcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCt                                 <  2:493628/139‑1 (MQ=255)

                                                                                             gcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCtt                                <  2:371221/139‑1 (MQ=255)

                                                                                                  aaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGccc                           <  1:132051/139‑1 (MQ=255)

                                                                                                           atcatcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                            <  2:346538/129‑1 (MQ=255)

                                                                                                           atcatcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGcc                            >  1:346538/1‑129 (MQ=255)

                                                                                                           atcatcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatga                  >  1:432454/1‑139 (MQ=255)

                                                                                                              atcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgat                 >  2:89753/1‑137 (MQ=255)

                                                                                                              atcTCCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAgatgat                 <  1:89753/137‑1 (MQ=255)

                                                                                                                 tcCAAAATCGAAAACCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCg            <  1:393934/139‑1 (MQ=255)

                                                                                                                           aaaaCCAGGAAGACCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAt  <  2:118905/139‑1 (MQ=255)

GTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAT  >  NC_000913/1756239‑1756500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A