Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	82	28.6	1225488	97.4%	1193625	116.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,464	T→C	V256A (GTT→GCT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,464	0	T	C	100.0%	96.9 / NA	26	V256A (GTT→GCT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/13); total (13/13)

GCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG  >  NC_000913/1756332‑1756598

gcgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCtt                                                                                                                                  <  1:103418/139‑1 (MQ=255)

 cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAATTCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTc                                                                                                                                 >  1:587417/1‑139 (MQ=255)

    aaaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc                                                                                                                              <  2:223160/139‑1 (MQ=255)

     aaCATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGccc                                                                                                                             <  1:268453/139‑1 (MQ=255)

       cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAATTCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCag                                                                                                                           <  2:587417/139‑1 (MQ=255)

                  tctcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCtt                                                                                                                                  >  2:518132/1‑121 (MQ=255)

                  tctcCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCtt                                                                                                                                  <  1:518132/121‑1 (MQ=255)

                                     ggAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCa                                                                                             >  2:160186/1‑139 (MQ=255)

                                                      tCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa                                                                                       >  1:418820/1‑128 (MQ=255)

                                                      tCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa                                                                                       <  2:418820/128‑1 (MQ=255)

                                                                  tCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGAt                                                                >  2:70212/1‑139 (MQ=255)

                                                                            tGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                          >  1:274749/1‑119 (MQ=255)

                                                                            tGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                          <  2:274749/119‑1 (MQ=255)

                                                                                   atcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGt                                                                                                >  2:159230/1‑90 (MQ=255)

                                                                                   atcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGt                                                                                                <  1:159230/90‑1 (MQ=255)

                                                                                     catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc                                                                                                                              <  1:6417/58‑1 (MQ=255)

                                                                                     catGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc                                                                                                                              >  2:6417/1‑58 (MQ=255)

                                                                                          ttGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc                                                                                                                              <  1:535459/53‑1 (MQ=255)

                                                                                          ttGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc                                                                                                                              >  2:535459/1‑53 (MQ=255)

                                                                                             cgcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACg                                     >  2:536372/1‑139 (MQ=255)

                                                                                              gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCg                                                                                                 >  2:420226/1‑78 (MQ=255)

                                                                                              gcgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCg                                                                                                 <  1:420226/78‑1 (MQ=255)

                                                                                                                     acccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa             >  2:375400/2‑139 (MQ=255)

                                                                                                                       ccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAt                                                                                                    >  2:11623/1‑50 (MQ=255)

                                                                                                                       ccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTAt                                                                                                    <  1:11623/50‑1 (MQ=255)

                                                                                                                                gaagCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg  <  1:70212/139‑1 (MQ=255)

GCGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG  >  NC_000913/1756332‑1756598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A