Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I2 R1	67	36.0	1614110	95.1%	1535018	114.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,464	T→C	V256A (GTT→GCT)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,464	0	T	C	100.0%	85.0 / NA	24	V256A (GTT→GCT)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (13/11); total (13/11)

CGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG  >  NC_000913/1756333‑1756598

cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGTTGTAGAAATCCCGGTAGAAGAAGCTAt                                                                                                                                     <  2:481441/135‑1 (MQ=255)

cgAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTACGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTAt                                                                                                                                     >  1:481441/1‑135 (MQ=255)

      cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGACGCTATCTTCGCCCag                                                                                                                           <  2:538813/139‑1 (MQ=255)

      cATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCag                                                                                                                           <  1:157007/139‑1 (MQ=255)

                                     gAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCAc                                                                                            >  2:797385/1‑139 (MQ=255)

                                          ccTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa                                                                                       <  1:797385/139‑1 (MQ=255)

                                          ccTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTaaa                                                                                       >  2:199726/1‑139 (MQ=255)

                                                      cgacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACt                                                                           >  2:106078/1‑139 (MQ=255)

                                                       gacgaAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTg                                                                          <  2:10994/139‑1 (MQ=255)

                                                               ccTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAg                                                                  <  1:199726/139‑1 (MQ=255)

                                                                                 catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGccc                                                                                                                             >  1:103348/1‑62 (MQ=255)

                                                                                 catcatGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGccc                                                                                                                             <  2:103348/62‑1 (MQ=255)

                                                                                        gTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAAcc                                         <  2:177600/139‑1 (MQ=255)

                                                                                           gcgcGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc                                                                                                                              >  1:444389/1‑51 (MQ=255)

                                                                                           gcgcGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGcc                                                                                                                              <  2:444389/51‑1 (MQ=255)

                                                                                              cgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCg                                                                                                              >  2:426207/1‑64 (MQ=255)

                                                                                              cgTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCg                                                                                                              <  1:426207/64‑1 (MQ=255)

                                                                                                tGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCg                                 >  2:742991/1‑139 (MQ=255)

                                                                                                   cGACCTGGGTGTAGAAATCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACt                              <  2:777274/139‑1 (MQ=255)

                                                                                                                    tCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa             >  2:585957/1‑139 (MQ=255)

                                                                                                                    tCCCGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGa             >  2:640447/1‑139 (MQ=255)

                                                                                                                      ccGGTAGAAGAAGCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACg           >  2:663616/1‑139 (MQ=255)

                                                                                                                             aagaagCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCaaa    >  1:441676/1‑139 (MQ=255)

                                                                                                                               gaagCTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACg  >  1:77122/1‑139 (MQ=255)

CGAAAACATCCACATCATCTCCAAAATCGAAAACCAGGAAGGCCTCAACAACTTCGACGAAATCCTCGAAGCCTCTGACGGCATCATGGTTGCGCGTGGCGACCTGGGTGTAGAAATCCCGGTAGAAGAAGTTATCTTCGCCCAGAAGATGATGATCGAAAAATGTATCCGTGCACGTAAAGTCGTTATCACTGCGACCCAGATGCTGGATTCCATGATCAAAAACCCACGCCCGACTCGCGCAGAAGCCGGTGACGTTGCAAACG  >  NC_000913/1756333‑1756598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A