Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F1 I2 R1
|
67 |
32.5 |
1435938 |
95.9% |
1377064 |
112.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,911,083 |
G→A |
S3S (AGC→AGT) |
mazF ← |
mRNA interferase toxin, antitoxin is MazE |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,911,083 | 0 | G | A | 82.1%
| 120.4
/ 22.6
| 56 | S3S (AGC→AGT) | mazF | mRNA interferase toxin, antitoxin is MazE |
| Reads supporting (aligned to +/- strand): ref base G (6/4); new base A (23/23); total (29/27) |
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 |
ACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAAT > NC_000913/2910946‑2911217
|
acacacAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc < 2:456137/139‑1 (MQ=255)
acacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACtt > 1:630510/1‑139 (MQ=255)
acacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACtt > 1:422468/1‑139 (MQ=255)
tACCTGTTTTGTTGTTTTACATGAAAGGACTCAGGAAAACAGCTGGACGATGTCAAGCTTGCTCGTTACCTTTGGTCGGGTAAAAATCAACCCAAATAAGATCGCCCATTTCGGGTACGTATCGACttaccattacaga < 1:292977/139‑4 (MQ=255)
tACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAg > 1:188046/1‑139 (MQ=255)
tACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAg < 2:188046/139‑1 (MQ=255)
aCCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGa > 2:642554/1‑139 (MQ=255)
gTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTc > 2:378385/1‑139 (MQ=255)
tttGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt > 1:577333/1‑139 (MQ=255)
ttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCttt < 1:161848/139‑1 (MQ=255)
tgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt > 2:711617/1‑131 (MQ=255)
tgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCt < 1:711617/131‑1 (MQ=255)
tGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGctct > 2:107023/1‑139 (MQ=255)
gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCtctc > 1:517234/1‑139 (MQ=255)
aaaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcc < 1:305140/139‑1 (MQ=255)
tCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGGCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCg < 2:630510/139‑1 (MQ=255)
aGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGat > 1:330989/1‑139 (MQ=255)
aGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGat > 2:650270/1‑139 (MQ=255)
caGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCg > 1:298249/1‑139 (MQ=255)
ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc > 1:89773/1‑119 (MQ=255)
ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAg > 2:119588/1‑139 (MQ=255)
ggACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACACAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc < 2:89773/119‑1 (MQ=255)
tGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc < 2:586213/113‑1 (MQ=255)
tGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTccc > 1:586213/1‑113 (MQ=255)
ttGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGa < 1:107023/139‑1 (MQ=255)
tCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTc > 1:307756/1‑139 (MQ=255)
tCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTc > 2:14658/1‑139 (MQ=255)
cGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCg < 2:557403/139‑1 (MQ=255)
cTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGtt < 2:422468/139‑1 (MQ=255)
aCCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGa < 1:650270/139‑1 (MQ=255)
aCCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGa < 2:330989/139‑1 (MQ=255)
tGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc < 1:313232/53‑1 (MQ=255)
tGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGc > 2:313232/1‑53 (MQ=255)
gggTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGc < 2:517234/139‑1 (MQ=255)
ggTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGc > 1:423841/1‑115 (MQ=255)
ggTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGc < 2:423841/115‑1 (MQ=255)
gTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCaa > 1:439876/1‑139 (MQ=255)
gTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCaa < 1:642554/139‑1 (MQ=255)
aaaTCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc < 1:378385/139‑1 (MQ=255)
aaaTCAGATCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc < 2:33817/139‑1 (MQ=255)
aTCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCCCTCCCCAGTCGATATTCTCGTGGAGGGTTTCCGGCGTGATGTCGTTGACCAGTTCAGCATGCGTAACTACGGGTTCTTTAc > 2:354187/1‑139 (MQ=255)
aTCGCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTAc > 2:655937/1‑139 (MQ=255)
gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcccc < 1:337179/61‑1 (MQ=255)
gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcccc > 2:337179/1‑61 (MQ=255)
gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCa < 2:307756/139‑1 (MQ=255)
gCCCATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCa < 2:298249/139‑1 (MQ=255)
ccATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACggg < 2:479380/126‑1 (MQ=255)
ccATATCGGGTACGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACggg > 1:479380/1‑126 (MQ=255)
cGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGt < 2:611379/50‑1 (MQ=255)
cGTATCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGt > 1:611379/1‑50 (MQ=255)
aTCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAAtaattaat > 1:641996/1‑139 (MQ=255)
aTCGACTTACCATTACCAGACTTCGTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACgg > 2:484124/1‑110 (MQ=255)
aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACgg < 1:484124/110‑1 (MQ=255)
aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc < 2:164704/112‑1 (MQ=255)
aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGc > 1:164704/1‑112 (MQ=255)
aTCGACTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAAtaattaat > 2:92105/1‑139 (MQ=255)
|
ACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTGATGTCGTTGACCAGTTCAGCAAGCGTAAATACGGGCTCTTTACGCACTGGCTCAATAATTAAT > NC_000913/2910946‑2911217
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
