Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I2 R1
|
92 |
50.6 |
2362856 |
94.3% |
2228173 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,933,613 |
T→C |
T26A (ACA→GCA) |
fucA ← |
L‑fuculose‑1‑phosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,933,613 | 0 | T | C | 100.0%
| 218.0
/ NA
| 59 | T26A (ACA→GCA) | fucA | L‑fuculose‑1‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/31); total (28/31) |
AGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCACACTAT > NC_000913/2933483‑2933743
|
aGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGt > 1:1056820/1‑139 (MQ=255)
gCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCTATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGtt < 2:769317/139‑1 (MQ=255)
tACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCAt < 1:594176/139‑1 (MQ=255)
ttGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCa > 1:1178622/1‑139 (MQ=255)
gCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAgg < 2:349760/139‑1 (MQ=255)
gCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAgg > 1:951660/1‑139 (MQ=255)
aTCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCaa < 2:1178622/139‑1 (MQ=255)
aTCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCaa < 1:440316/139‑1 (MQ=255)
aTCAACAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCaa < 2:733218/139‑1 (MQ=255)
tCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAg < 2:320371/139‑1 (MQ=255)
atatGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCcccc < 2:398705/93‑1 (MQ=255)
atatGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCcccc > 1:398705/1‑93 (MQ=255)
atatGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCt > 2:355599/1‑139 (MQ=255)
gACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAg < 2:774357/139‑1 (MQ=255)
aCTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAgtgt > 1:1035349/1‑123 (MQ=255)
aCTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAgtgt < 2:1035349/123‑1 (MQ=255)
aCTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGc > 1:527105/1‑139 (MQ=255)
ccGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAg > 2:90826/1‑135 (MQ=255)
ccGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAg < 1:90826/135‑1 (MQ=255)
cGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTc < 1:148304/103‑1 (MQ=255)
cGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTc > 2:148304/1‑103 (MQ=255)
tCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGttt > 1:50768/1‑139 (MQ=255)
tCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGttt > 2:888586/1‑139 (MQ=255)
ttCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGt > 2:36729/1‑139 (MQ=255)
gAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAg < 1:355599/139‑1 (MQ=255)
aaTGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGc > 1:947042/1‑139 (MQ=255)
aTGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAgg < 2:290005/78‑1 (MQ=255)
aTGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAgg > 1:290005/1‑78 (MQ=255)
tGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTa < 2:50768/139‑1 (MQ=255)
tAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACTAGCAAGTTTATTTCGt > 1:727228/1‑122 (MQ=255)
tAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACTAGCAAGTTTATTTCGt < 2:727228/122‑1 (MQ=255)
aaTCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGAtt > 2:381458/1‑139 (MQ=255)
aTCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCg > 1:457803/1‑113 (MQ=255)
aTCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCg < 2:457803/113‑1 (MQ=255)
catcccatccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGt < 1:333631/63‑1 (MQ=255)
catcccatccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGt > 2:333631/1‑63 (MQ=255)
atcccatccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACa < 1:379396/139‑1 (MQ=255)
aTAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTc < 1:233571/99‑1 (MQ=255)
aTAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTc > 2:233571/1‑99 (MQ=255)
cGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAg > 1:1118184/1‑79 (MQ=255)
cGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAg < 2:1118184/79‑1 (MQ=255)
gTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATgttgtt < 2:951660/139‑1 (MQ=255)
acacTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTcc > 1:342624/1‑139 (MQ=255)
cacTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGCCGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCaaaa > 2:1041731/1‑118 (MQ=255)
cacTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCaaaa < 1:1041731/118‑1 (MQ=255)
acTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCtt < 2:947042/139‑1 (MQ=255)
cTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACctctctc < 2:567080/106‑1 (MQ=255)
cTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACctctctc > 1:567080/1‑106 (MQ=255)
cTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCttt < 1:1114738/139‑1 (MQ=255)
tGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTAcc > 1:1173376/1‑99 (MQ=255)
tGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTAcc < 2:1173376/99‑1 (MQ=255)
tGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACctctctc < 2:351530/105‑1 (MQ=255)
tGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACctctctc > 1:351530/1‑105 (MQ=255)
tGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCaa > 2:520983/1‑123 (MQ=255)
tGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCaa < 1:520983/123‑1 (MQ=255)
gACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTca < 2:527105/139‑1 (MQ=255)
gACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTca < 2:342624/139‑1 (MQ=255)
aCGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTcac > 1:650574/1‑139 (MQ=255)
ccccGCTGCCCCCTGGTTCAGTCCGAGGCGGGGCATTTCCAGGCAAGTGTCACTAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGAGTCAAAACAGGGCAATAATGTTGTTCCTTTCACACTAt > 1:798021/1‑139 (MQ=255)
|
AGCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCACACTAT > NC_000913/2933483‑2933743
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
