Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
86 |
24.1 |
1002126 |
98.1% |
983085 |
121.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,933,613 |
T→C |
T26A (ACA→GCA) |
fucA ← |
L‑fuculose‑1‑phosphate aldolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,933,613 | 0 | T | C | 100.0%
| 50.1
/ NA
| 15 | T26A (ACA→GCA) | fucA | L‑fuculose‑1‑phosphate aldolase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (8/7); total (8/7) |
GCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCAC > NC_000913/2933484‑2933738
|
gCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGtt < 1:389760/139‑1 (MQ=255)
accATCAATAAAGACAATATGCGACTCCGTCAGTTTTGCATATGGAATGCCTGTAGGCGTGATCAGCAGCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAgg < 1:47391/138‑1 (MQ=255)
tCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAg < 2:432273/139‑1 (MQ=255)
atatGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGtt > 1:116362/1‑98 (MQ=255)
atatGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGtt < 2:116362/98‑1 (MQ=255)
tCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTc < 1:282518/105‑1 (MQ=255)
tCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTc > 2:282518/1‑105 (MQ=255)
aTCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTc > 2:56891/1‑139 (MQ=255)
ctccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGA‑TCAAAACAGGGCaa < 1:8332/138‑1 (MQ=255)
ccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCaat > 1:263001/1‑139 (MQ=255)
ccTGATAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCaat > 2:328267/1‑139 (MQ=255)
aTAACGTACACTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAAtg > 2:348397/1‑139 (MQ=255)
cTGACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCttt > 1:10235/1‑139 (MQ=255)
gACGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTca < 1:58412/139‑1 (MQ=255)
aCGTTCCCCGCTGCCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTcac > 1:85116/1‑139 (MQ=255)
|
GCTTTCCTTCCTCATGTTTACCGTTGCCATCAATAAAGACAATATGCGACTCCGTCAGTTTTTCATATGGAATGCCTGTAGGCGTAATCAGCATCCCATCCTGATAACGTACACTGACGTTCCCCGCTGTCCCCTGGTTCAGTCCCAGGCGGGTCATTTCCAGGCAAGTGTCAATAATCTGACGAGCAAGTTTATTTCGTTCCATTAGCTACCTCTCTCTGATTCAAAACAGGGCAATAATGTTGTTCCTTTCAC > NC_000913/2933484‑2933738
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
