Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F1 I1 R1	86	24.1	1002126	98.1%	983085	121.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,634,940	A→G	S34G (AGC→GGC)	yhiM →	acid resistance protein, inner membrane

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,634,940	0	A	G	100.0%	128.1 / NA	34	S34G (AGC→GGC)	yhiM	acid resistance protein, inner membrane
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/19); total (15/19)

ATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTT  >  NC_000913/3634811‑3635054

atatTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTAtt                                                                                                           <  1:470724/139‑1 (MQ=255)

      gCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTtcgtcg                                                                                                     >  1:349523/1‑139 (MQ=255)

      gCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTtcgtcg                                                                                                     >  2:213156/1‑139 (MQ=255)

       gaGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTcgc                                                                                                    >  1:312001/2‑139 (MQ=255)

            aTACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTc                                                                                               <  2:172289/139‑1 (MQ=255)

               ccTGAGGAGCTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCAtg                                                                                            >  2:276076/1‑139 (MQ=255)

                   aggagTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCt                                                                                        >  2:482902/1‑139 (MQ=255)

                          tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                 >  1:141531/1‑139 (MQ=255)

                          tAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTtct                                                                                 >  2:479206/1‑139 (MQ=255)

                                gAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcg                                                                           >  1:203785/1‑139 (MQ=255)

                                gAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcg                                                                           <  2:430786/139‑1 (MQ=255)

                                 aaCATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcgg                                                                          >  1:150956/1‑139 (MQ=255)

                                 aaCATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTggcgg                                                                          >  2:173551/1‑139 (MQ=255)

                                          atCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                 <  2:217833/139‑1 (MQ=255)

                                          atCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                 <  1:479206/139‑1 (MQ=255)

                                          atCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGcc                                                                 <  1:401203/139‑1 (MQ=255)

                                             gggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTgg                                                              <  2:410054/139‑1 (MQ=255)

                                              ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTTGCGGCCATATGCCTGGc                                                             >  2:379377/1‑139 (MQ=255)

                                              ggTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGc                                                             >  2:357005/1‑139 (MQ=255)

                                                                   cTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATttat                                        <  2:1264/139‑1 (MQ=255)

                                                                        aTTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCa                                   <  1:8401/139‑1 (MQ=255)

                                                                            tGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTc                               >  2:198803/1‑139 (MQ=255)

                                                                                cTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAg                           >  2:189650/1‑139 (MQ=255)

                                                                                        gTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                   <  2:141531/139‑1 (MQ=255)

                                                                                         tATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcgc                  <  1:482902/139‑1 (MQ=255)

                                                                                           tCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                   <  2:229773/136‑1 (MQ=255)

                                                                                           tCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCAcgcg                   >  1:229773/1‑136 (MQ=255)

                                                                                                ttAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTa           <  1:76336/139‑1 (MQ=255)

                                                                                                  aGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAAt         <  1:276076/139‑1 (MQ=255)

                                                                                                     tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg      <  2:150956/139‑1 (MQ=255)

                                                                                                     tGGCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACg      <  2:388689/139‑1 (MQ=255)

                                                                                                       gCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtt    <  2:349523/139‑1 (MQ=255)

                                                                                                       gCTTTGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtt    <  1:189650/139‑1 (MQ=255)

                                                                                                         tttGTGCTGGAAAGTTCAGGGCAAGGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGtttt  <  1:213156/139‑1 (MQ=255)

ATATTAGCAGGGATACCTGAGGAGTTTAAAGTGAACATATATATCGGGTGGCTTTTCAAATTAATCCCTTTGATTATGGGCTTAATTTGTATCGCGTTAGGTGGCTTTGTGCTGGAAAGTTCAGGGCAAAGCGAGTATTTCGTCGCGGGTCATGTGCTGATTTCTCTGGCGGCCATATGCCTGGCATTATTCACTACCGCATTTATTATCATTTCGCAGCTCACGCGCGGCGTTAATACGTTTT  >  NC_000913/3634811‑3635054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A