Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F1 I1 R1
|
86 |
24.1 |
1002126 |
98.1% |
983085 |
121.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,655,564 |
G→A |
S113S (AGC→AGT) |
yhiD ← |
putative Mg(2+) transport ATPase, inner membrane protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,655,564 | 0 | G | A | 100.0%
| 84.5
/ NA
| 25 | S113S (AGC→AGT) | yhiD | putative Mg(2+) transport ATPase, inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/11); total (14/11) |
TACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCAAT > NC_000913/3655432‑3655698
|
tACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCa < 2:490827/139‑1 (MQ=255)
cGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATa > 2:73494/1‑139 (MQ=255)
gTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATaa < 1:461839/139‑1 (MQ=255)
ttACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCa > 2:500066/1‑139 (MQ=255)
ttACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCa > 2:14980/1‑139 (MQ=255)
ttCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTa > 2:64335/1‑139 (MQ=255)
cAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTc < 1:493871/139‑1 (MQ=255)
aGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCa > 1:372629/1‑139 (MQ=255)
cGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTa < 2:372629/139‑1 (MQ=255)
ggAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTacg > 1:96139/1‑139 (MQ=255)
ggTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTa < 1:195417/101‑1 (MQ=255)
ggTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTa > 2:195417/1‑101 (MQ=255)
gTCATCACTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGa > 2:132381/1‑139 (MQ=255)
atcaCTGAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATaa > 1:283261/1‑139 (MQ=255)
gAACCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGa < 2:472393/139‑1 (MQ=255)
aaCCATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGAc > 1:445307/1‑139 (MQ=255)
ccATAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGc < 1:132381/139‑1 (MQ=255)
tAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCg > 1:82065/1‑139 (MQ=255)
tAAATACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCg > 2:494399/1‑139 (MQ=255)
aaTACCCAGTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTc < 2:445307/139‑1 (MQ=255)
gTTCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTg < 1:64335/139‑1 (MQ=255)
ttCGTACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGa > 1:437566/1‑139 (MQ=255)
tACATACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATGTTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGagcag > 1:191163/1‑139 (MQ=255)
aTACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCaa < 2:375243/139‑1 (MQ=255)
tACCACTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCAAt < 2:437566/139‑1 (MQ=255)
|
TACCGTATTACCGTTCACTAATGTCAGCTGTAAATGGTAATTTTTGTTCATCAGGCGGAAGGTTAATTGATGGAAGACTTCCAGCACCAACAAGGTCATCACTGAACCATAAATACCCAGTTCGTACATACCGCTGCCAATAACCATACCTATGGCGGCGGTCACCCAGATATCCGCTGCCGTCGTCAGACCTACAATATTTTGGTTACGAACGAGAATGTTACCTGCACCGATAAACCCGACGCCCGTCACCACCTGAGCAGCAAT > NC_000913/3655432‑3655698
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
