Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A10 F1 I2 R1 67 36.0 1614110 95.1% 1535018 114.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,581,323 G→A T401I (ACA→ATA)  ydeN ← putative Ser‑type periplasmic non‑aryl sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,581,3230GA100.0% 74.5 / NA 22T401I (ACA→ATA) ydeNputative Ser‑type periplasmic non‑aryl sulfatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/11);  total (11/11)

TATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTGTCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTAT  >  NC_000913/1581205‑1581437
                                                                                                                      |                                                                                                                  
tATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCg                                                                                                >  2:68080/1‑139 (MQ=255)
tATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCg                                                                                                >  1:617564/1‑139 (MQ=255)
          aGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCt                                                                                      >  1:350585/1‑139 (MQ=255)
            aTTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTg                                                                                    <  1:68080/139‑1 (MQ=255)
                      ggCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       >  1:241023/1‑126 (MQ=255)
                      ggCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       <  2:241023/126‑1 (MQ=255)
                      ggCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTAc                                                                          >  1:661971/1‑139 (MQ=255)
                                       aTCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTtttcct                                                         >  2:638627/1‑139 (MQ=255)
                                            gCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTcca                                                    <  1:638627/139‑1 (MQ=255)
                                             cAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTccac                                                   <  2:617564/139‑1 (MQ=255)
                                              aaCCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTccacc                                                  >  1:552489/1‑139 (MQ=255)
                                                        gcaAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACAt                                        >  1:389849/1‑139 (MQ=255)
                                                         caAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACAtt                                       <  2:350585/139‑1 (MQ=255)
                                                         caAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACAtt                                       <  2:424577/139‑1 (MQ=255)
                                                                aCGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGgtgt                                <  1:224791/139‑1 (MQ=255)
                                                                    cATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGt                            <  2:389849/139‑1 (MQ=255)
                                                                    cATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGt                            <  2:552489/139‑1 (MQ=255)
                                                                              aaGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGa                  <  2:661971/139‑1 (MQ=255)
                                                                                ggTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       >  2:195894/1‑68 (MQ=255)
                                                                                ggTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGc                                                                                       <  1:195894/68‑1 (MQ=255)
                                                                                            tGCTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCtt    >  1:272458/1‑139 (MQ=255)
                                                                                              cTGATATCGGCTGCATCAAGAGCTATCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTtat  >  1:131639/1‑139 (MQ=255)
                                                                                                                      |                                                                                                                  
TATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTGATATCGGCTGCATCAAGAGCTGTCGGGTAGAAATCCATTGCGGAAATCAGCTTGTCATAATTACCGGGTTGAAGTTTTCCTTTCCACCACATAAACATTGGGGTGTGAGTACCGCCAGGATAGGTCTGACTCTTAT  >  NC_000913/1581205‑1581437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A