Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	83	73.5	3575638	92.3%	3300313	107.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,343	A→G	Y167C (TAC→TGC)	purR →	transcriptional repressor, hypoxanthine‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,343	0	A	G	100.0%	175.0 / NA	46	Y167C (TAC→TGC)	purR	transcriptional repressor, hypoxanthine‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/20); total (26/20)

TGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGT  >  NC_000913/1738215‑1738475

tGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCggg                                                                                                                            <  1:603408/139‑1 (MQ=255)

                ttGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAAc                                                                                                            >  2:940376/1‑139 (MQ=255)

                               gAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                      >  2:1055789/1‑114 (MQ=255)

                               gAAGAGTATCGCCATATCCCAATGGTGGTCATAGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTAt                                                                                                                      <  1:1055789/114‑1 (MQ=255)

                                                   aaTGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCg                                                                         >  2:1452397/1‑139 (MQ=255)

                                                         ggtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGaa                                                                   >  1:265582/1‑139 (MQ=255)

                                                          gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTGATTGATAACGCGTTCGAAGGCGGCTGCATGGCCgggt                                                                                                                           <  1:1194775/82‑2 (MQ=255)

                                                          gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAAc                                                                  >  2:1713486/1‑139 (MQ=255)

                                                          gtCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATAGCCgggt                                                                                                                           >  2:1194775/1‑81 (MQ=255)

                                                              tGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTaa                                                              <  2:26873/139‑1 (MQ=255)

                                                                 aCTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTTacac                                                           <  1:1038271/139‑1 (MQ=255)

                                                                     gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      >  2:941014/1‑108 (MQ=255)

                                                                     gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      <  1:941014/108‑1 (MQ=255)

                                                                     gggTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGgc                                                       >  1:221978/1‑139 (MQ=255)

                                                                           aGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCAGCGCAGgc                                                 >  2:1187672/1‑139 (MQ=255)

                                                                               aaaGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      <  1:179036/98‑1 (MQ=255)

                                                                               aaaGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGt                                                                                      >  2:179036/1‑98 (MQ=255)

                                                                               aaaGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGgccgcc                                             >  1:57171/1‑139 (MQ=255)

                                                                                      aCTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGc                                         <  2:707835/136‑1 (MQ=255)

                                                                                      aCTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGc                                         >  1:707835/1‑136 (MQ=255)

                                                                                       cTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                          >  1:110975/1‑54 (MQ=255)

                                                                                       cTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCg                                                                                                                          <  2:110975/54‑1 (MQ=255)

                                                                                       cTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGt                                     >  1:1162550/1‑139 (MQ=255)

                                                                                             cGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATg                               <  2:221978/139‑1 (MQ=255)

                                                                                                tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGt                                     >  2:1458711/1‑130 (MQ=255)

                                                                                                tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGt                                     <  1:1458711/130‑1 (MQ=255)

                                                                                                tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg                            <  2:57171/139‑1 (MQ=255)

                                                                                                tGCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAg                            >  1:451829/1‑139 (MQ=255)

                                                                                                 gCGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCACCTTGCCGGTTTTATGAAgg                           <  1:1713486/139‑1 (MQ=255)

                                                                                                  cGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCgg                                      >  1:740623/1‑127 (MQ=255)

                                                                                                  cGGTCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCgg                                      <  2:740623/127‑1 (MQ=255)

                                                                                                     tCATTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGAt                       <  2:265582/139‑1 (MQ=255)

                                                                                                       aTTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCg                                                                              >  1:1558864/1‑82 (MQ=255)

                                                                                                       aTTGATAACGCGTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCg                                                                              <  2:1558864/82‑1 (MQ=255)

                                                                                                               cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGa                              >  1:63341/1‑122 (MQ=255)

                                                                                                               cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGa                              <  2:63341/122‑1 (MQ=255)

                                                                                                               cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaagaa                <  1:152359/136‑1 (MQ=255)

                                                                                                               cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaagaa                >  2:152359/1‑136 (MQ=255)

                                                                                                               cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCg             >  1:783558/1‑139 (MQ=255)

                                                                                                               cgcgTTCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCg             >  1:1757183/1‑139 (MQ=255)

                                                                                                                   ttCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaag                  <  2:757522/130‑1 (MQ=255)

                                                                                                                   ttCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGgaag                  >  1:757522/1‑130 (MQ=255)

                                                                                                                   ttCGAAGGCGGCTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCgatga         >  1:595162/1‑139 (MQ=255)

                                                                                                                          gcggcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCGCCg                                                                              >  1:1178782/1‑63 (MQ=255)

                                                                                                                          gcggcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCg                                                                              <  2:1178782/63‑1 (MQ=255)

                                                                                                                          gcggcTGCATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGt  >  1:136856/1‑139 (MQ=255)

TGAGTACCCAGAGCCGTTGCTGGCGATGCTGGAAGAGTATCGCCATATCCCAATGGTGGTCATGGACTGGGGTGAAGCAAAAGCTGACTTCACCGATGCGGTCATTGATAACGCGTTCGAAGGCGGCTACATGGCCGGGCGTTATCTGATTGAACGCGGTCACCGCGAAATCGGCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGT  >  NC_000913/1738215‑1738475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A