Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I1 R1	83	73.5	3575638	92.3%	3300313	107.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,133	C→T	V185I (GTC→ATC)	mazG ←	nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,133	0	C	T	100.0%	202.5 / NA	58	V185I (GTC→ATC)	mazG	nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (25/33); total (25/33)

CGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCG  >  NC_000913/2910001‑2910267

cgcgAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGc                                                                                                                                  >  1:1551938/1‑139 (MQ=255)

cgcgAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGc                                                                                                                                  <  2:869237/139‑1 (MQ=255)

       acgacgCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgc                                                                                                                           >  1:1715715/1‑139 (MQ=255)

          acgCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCt                                                                                                                        >  1:531535/1‑139 (MQ=255)

          acgCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCt                                                                                                                        <  1:1328741/139‑1 (MQ=255)

                      ttcgttcgCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCAcc                                                                                                            <  1:1013467/139‑1 (MQ=255)

                              cTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt                                                                                                    <  2:1715715/139‑1 (MQ=255)

                              cTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt                                                                                                    <  2:531535/139‑1 (MQ=255)

                               tttttGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCGGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  1:182804/139‑1 (MQ=255)

                               tttttGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  2:709223/139‑1 (MQ=255)

                                     cAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCg                                                                                             <  2:1551938/139‑1 (MQ=255)

                                                cTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc                                                                                  <  2:1283880/139‑1 (MQ=255)

                                                cTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc                                                                                  <  1:169653/139‑1 (MQ=255)

                                                      tCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTAc                                                                            <  2:1082591/139‑1 (MQ=255)

                                                       cGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTa                                                                                           >  2:229152/1‑123 (MQ=255)

                                                       cGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATCTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTa                                                                                           <  1:229152/123‑1 (MQ=255)

                                                             tAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCa                                                                     >  2:1203979/1‑139 (MQ=255)

                                                                 tGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcg                                                                 <  2:1467744/139‑1 (MQ=255)

                                                                    cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          <  1:598448/79‑1 (MQ=255)

                                                                    cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          >  2:598448/1‑79 (MQ=255)

                                                                    cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAACCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtcg                                                              >  2:1652664/1‑139 (MQ=255)

                                                                      aGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCgtcgtc                                                            <  2:1394496/139‑1 (MQ=255)

                                                                                 ccGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGcc                                                 >  1:1687950/1‑139 (MQ=255)

                                                                                     ggCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          >  1:1507826/1‑62 (MQ=255)

                                                                                     ggCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          <  2:1507826/62‑1 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTg                                                                                                                               <  2:970107/56‑1 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTg                                                                                                                               >  1:970107/1‑56 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCtt                                                                                                                       <  1:176942/64‑1 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCtt                                                                                                                       >  2:176942/1‑64 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCAcc                                                                                                            >  1:1564111/1‑75 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCAcc                                                                                                            <  2:1564111/75‑1 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtct                                                                                                  <  1:636845/85‑1 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtct                                                                                                  >  2:636845/1‑85 (MQ=255)

                                                                                      gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGt                                            >  1:1181702/1‑139 (MQ=255)

                                                                                           cagcagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   >  2:327688/1‑79 (MQ=255)

                                                                                           cagcagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  1:327688/79‑1 (MQ=255)

                                                                                            agcagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGgcgc                                      <  1:32683/139‑1 (MQ=255)

                                                                                                 gTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGCTAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGATCAAGCGTCGTCCAATCGAAGcc                                                 <  2:1131610/123‑1 (MQ=255)

                                                                                                 gTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTGGTAGACTTTATCGACTACCGGATCAAGCGTCGTCCAATCGAAGcc                                                 >  1:1131610/1‑123 (MQ=255)

                                                                                                   cccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          >  1:142149/1‑48 (MQ=255)

                                                                                                   cccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          <  2:142149/48‑1 (MQ=255)

                                                                                                    ccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGcc                                                 <  1:1595238/120‑1 (MQ=255)

                                                                                                    ccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGcc                                                 >  2:1595238/1‑120 (MQ=255)

                                                                                                              tcctccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCtcgtc                                                                                                       >  1:60412/1‑56 (MQ=255)

                                                                                                              tcctccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCtcgtc                                                                                                       <  2:60412/56‑1 (MQ=255)

                                                                                                               cctccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGATCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTTTGGATTTtgtg                   <  1:1034875/139‑1 (MQ=255)

                                                                                                                   cAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               <  1:1577128/139‑1 (MQ=255)

                                                                                                                   cAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               <  1:1165646/139‑1 (MQ=255)

                                                                                                                       ttAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgcacgca           >  1:929285/1‑139 (MQ=255)

                                                                                                                        tAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               >  2:1653547/1‑134 (MQ=255)

                                                                                                                        tAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               <  1:1653547/134‑1 (MQ=255)

                                                                                                                           ccTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAATGTTTCTGGATTTTTTGCGCACGCATTaa       >  2:1070438/1‑139 (MQ=255)

                                                                                                                             tGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAg     <  2:293782/139‑1 (MQ=255)

                                                                                                                               gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg                                                      >  2:139653/1‑88 (MQ=255)

                                                                                                                               gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCg                                                      <  1:139653/88‑1 (MQ=255)

                                                                                                                               gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGcc   >  1:1038748/1‑139 (MQ=255)

                                                                                                                                tCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGTGCACGCATTAAAGCCg  >  2:1299885/1‑139 (MQ=255)

                                                                                                                                tCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg  <  2:1038748/139‑1 (MQ=255)

CGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCG  >  NC_000913/2910001‑2910267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A