Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	94	59.5	3027142	91.9%	2781943	108.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,910,133	C→T	V185I (GTC→ATC)	mazG ←	nucleoside triphosphate pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,910,133	0	C	T	100.0%	189.6 / NA	55	V185I (GTC→ATC)	mazG	nucleoside triphosphate pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/26); total (29/26)

TTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCG  >  NC_000913/2909999‑2910267

ttCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGataaca                                                                                                                                    <  2:1480967/139‑1 (MQ=255)

  cgcgAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGc                                                                                                                                  >  1:1090861/1‑139 (MQ=255)

  cgcgAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGc                                                                                                                                  >  2:207384/1‑139 (MQ=255)

     gAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTg                                                                                                                               >  2:1113870/1‑139 (MQ=255)

       aaaCGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGcc                                                                                                                             <  1:419047/139‑1 (MQ=255)

          acgcccttcaaTTTTTCCTTCCCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCcgcg                                                                                                                          <  2:792843/130‑1 (MQ=255)

                                 tttttGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  2:1410575/139‑1 (MQ=255)

                                 tttttGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  1:515606/139‑1 (MQ=255)

                                         aTGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTa                                                                                           <  1:207384/139‑1 (MQ=255)

                                           gCGATTTTTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGa                                                                                         >  2:988433/1‑139 (MQ=255)

                                           gCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGa                                                                                         <  1:1113870/139‑1 (MQ=255)

                                                ttCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTa                                                                                    <  2:1090861/139‑1 (MQ=255)

                                                    gCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGa                                                                                >  1:1360478/1‑139 (MQ=255)

                                                             ccTAAATGGCGAGCCAGATTAACCGTGACAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGAc                                                                       >  1:1478399/1‑139 (MQ=255)

                                                                     gCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtc                                                               >  2:784548/1‑139 (MQ=255)

                                                                      cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtcg                                                              >  1:1151629/1‑139 (MQ=255)

                                                                      cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGcgtcg                                                              >  2:62943/1‑139 (MQ=255)

                                                                      cGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGAACCAAGcgtcg                                                              >  1:976807/1‑139 (MQ=255)

                                                                        aGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   <  2:876395/100‑1 (MQ=255)

                                                                        aGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAtc                                                                                                   >  1:876395/1‑100 (MQ=255)

                                                                        aGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCCGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCgtcgtc                                                            <  1:802643/139‑1 (MQ=255)

                                                                                   ccGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGcc                                                 <  1:625735/139‑1 (MQ=255)

                                                                                       ggCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTc                                                                                                                      <  2:1106483/66‑1 (MQ=255)

                                                                                       ggCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTc                                                                                                                      >  1:1106483/1‑66 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCt                                                                                                                        <  2:231196/63‑1 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCt                                                                                                                        >  1:231196/1‑63 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc                                                                                  <  1:991252/101‑1 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATc                                                                                  >  2:991252/1‑101 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGAc                                                                       >  1:1266563/1‑112 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGAc                                                                       <  2:1266563/112‑1 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAAt                                                        >  1:118623/1‑127 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAAt                                                        <  2:118623/127‑1 (MQ=255)

                                                                                        gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGt                                            >  1:872393/1‑139 (MQ=255)

                                                                                               gcagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCa                                     <  2:1360478/139‑1 (MQ=255)

                                                                                                cagGTCCCCCATTTCCTCCTCCGGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCaa                                    <  1:531965/139‑1 (MQ=255)

                                                                                                cagGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCaa                                    <  2:872393/139‑1 (MQ=255)

                                                                                                   gTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGt                                 >  1:1090735/1‑139 (MQ=255)

                                                                                                     cccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGttt                               >  1:925321/1‑139 (MQ=255)

                                                                                                      ccccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTc                              >  2:820145/1‑139 (MQ=255)

                                                                                                        ccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAg                                                                                          <  1:458047/77‑1 (MQ=255)

                                                                                                        ccATTTCCTCCTCCAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAg                                                                                          >  2:458047/1‑77 (MQ=255)

                                                                                                                 cctccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTAc                                                                            <  2:97041/82‑1 (MQ=255)

                                                                                                                 cctccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTAc                                                                            >  1:97041/1‑82 (MQ=255)

                                                                                                                   tccAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTgcg                 >  2:306192/1‑139 (MQ=255)

                                                                                                                     cAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               <  2:1151629/139‑1 (MQ=255)

                                                                                                                     cAGTTTAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca               <  1:784548/139‑1 (MQ=255)

                                                                                                                        tttAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt                                                                                                    >  2:1057192/1‑51 (MQ=255)

                                                                                                                        tttAGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGAt                                                                                                    <  1:1057192/51‑1 (MQ=255)

                                                                                                                           aGCCTGGTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCAtt         <  2:1478399/139‑1 (MQ=255)

                                                                                                                             ccTGCTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa       <  1:993371/139‑1 (MQ=255)

                                                                                                                                 gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa       <  2:266132/135‑1 (MQ=255)

                                                                                                                                 gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTaa       >  1:266132/1‑135 (MQ=255)

                                                                                                                                 gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGcc   >  2:320371/1‑139 (MQ=255)

                                                                                                                                 gTCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGcc   >  2:902113/1‑139 (MQ=255)

                                                                                                                                  tCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGGCTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg  >  2:358062/1‑139 (MQ=255)

                                                                                                                                  tCGATAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg  >  1:1273970/1‑139 (MQ=255)

TTCGCGAAAACGACGCTCGAATTTTTCGTTCGCTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCG  >  NC_000913/2909999‑2910267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A