Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F1 I1 R1 83 73.5 3575638 92.3% 3300313 107.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,450,125 T→C T37A (ACC→GCC)  rplV ← 50S ribosomal subunit protein L22

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,450,1250TC100.0% 305.0 / NA 81T37A (ACC→GCC) rplV50S ribosomal subunit protein L22
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/42);  total (39/42)

CTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCT  >  NC_000913/3449992‑3450255
                                                                                                                                     |                                                                                                                                  
cTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaaaa                                                                                                                               <  2:1453050/139‑1 (MQ=255)
cTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaaaa                                                                                                                               <  2:745938/139‑1 (MQ=255)
cTCGGGCCTTCGTCAACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaaaa                                                                                                                               <  2:1232850/139‑1 (MQ=255)
    ggCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATc                                                                                                                           >  1:1238717/1‑139 (MQ=255)
      ccTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                         <  1:1303527/139‑1 (MQ=255)
      ccTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                         >  1:866784/1‑139 (MQ=255)
        ttCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCaga                                                                                                                       >  1:1696001/1‑139 (MQ=255)
        ttCGTCTACGAAAATTTTCATAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCaga                                                                                                                       >  2:930150/1‑139 (MQ=255)
            tCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCt                                                                                                                   <  1:790212/139‑1 (MQ=255)
             cTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTg                                                                                                                  >  2:484795/1‑139 (MQ=255)
              tACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGc                                                                                                                 <  2:1674250/139‑1 (MQ=255)
                cGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTTGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               <  2:1497189/139‑1 (MQ=255)
                cGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               <  2:586474/139‑1 (MQ=255)
                cGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               <  1:1545013/139‑1 (MQ=255)
                cGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               <  2:1546974/139‑1 (MQ=255)
                        ttCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCt                                                                                                       <  1:484795/139‑1 (MQ=255)
                                       tCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGt                                                                                        <  2:1696001/139‑1 (MQ=255)
                                          tCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               <  2:270189/113‑1 (MQ=255)
                                          tCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               >  1:270189/1‑113 (MQ=255)
                                          tCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               >  2:1333417/1‑113 (MQ=255)
                                          tCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               <  1:1333417/113‑1 (MQ=255)
                                          tCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGt                                                                                        >  1:1755887/1‑136 (MQ=255)
                                          tCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGt                                                                                        <  2:1755887/136‑1 (MQ=255)
                                          tCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAg                                                                                     >  2:1216129/1‑139 (MQ=255)
                                             aTGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaa                                                                                  <  2:1212738/139‑1 (MQ=255)
                                             aTGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaa                                                                                  <  2:1447662/139‑1 (MQ=255)
                                                  aGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCTGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAgg                                                                             <  1:330164/139‑1 (MQ=255)
                                                  aGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGaca                                                                                                             <  2:1191610/107‑1 (MQ=255)
                                                  aGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGaca                                                                                                             >  1:1191610/1‑107 (MQ=255)
                                                    cgcCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATc                                                                                            <  1:614743/122‑1 (MQ=255)
                                                    cgcCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATc                                                                                            >  2:614743/1‑122 (MQ=255)
                                                     gcCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTGTTGACCAGTACAGCCGCCTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGCCGa                                                                          <  1:1148760/139‑1 (MQ=255)
                                                     gcCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                          <  1:72970/139‑1 (MQ=255)
                                                        aTCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaa                                                                                  >  1:1165392/1‑128 (MQ=255)
                                                        aTCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaa                                                                                  <  2:1165392/128‑1 (MQ=255)
                                                        aTCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAAcc                                                                       >  1:249604/1‑139 (MQ=255)
                                                          cGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCtt                                                                     <  2:1677908/139‑1 (MQ=255)
                                                            ttGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCt                                                                   >  2:1439449/1‑139 (MQ=255)
                                                             tgtgTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTg                                                                  <  2:42694/139‑1 (MQ=255)
                                                              gtgtTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCt                                                                                                                   <  2:1743436/89‑1 (MQ=255)
                                                              gtgtTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCt                                                                                                                   >  1:1743436/1‑89 (MQ=255)
                                                                   cAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCaga                                                            >  2:1458393/1‑139 (MQ=255)
                                                                           gCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                    >  1:1571046/1‑139 (MQ=255)
                                                                           gCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                    <  2:503798/139‑1 (MQ=255)
                                                                             aaTGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCa                                                  <  1:218020/139‑1 (MQ=255)
                                                                                ggCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCagaaga                                                         <  2:678110/129‑1 (MQ=255)
                                                                                ggCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCagaaga                                                         >  1:678110/1‑129 (MQ=255)
                                                                                ggCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATgg                                               >  2:929135/1‑139 (MQ=255)
                                                                                  cAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCg                                             <  2:249604/139‑1 (MQ=255)
                                                                                     aTTCCAGAACTTTCTTGACCAGTACAGCCTCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGa                                                                 <  2:1182655/116‑1 (MQ=255)
                                                                                     aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               >  1:380381/1‑70 (MQ=255)
                                                                                     aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                               <  2:380381/70‑1 (MQ=255)
                                                                                     aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                          >  1:17857/1‑107 (MQ=255)
                                                                                     aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                          <  2:17857/107‑1 (MQ=255)
                                                                                     aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATACCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGa                                                                 >  1:1182655/1‑116 (MQ=255)
                                                                                           gAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaac                                                                                 >  1:1303666/1‑94 (MQ=255)
                                                                                           gAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaac                                                                                 <  2:1303666/94‑1 (MQ=255)
                                                                                               tttCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATa                                <  1:1458393/139‑1 (MQ=255)
                                                                                                 tCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGt                              >  2:1067050/1‑139 (MQ=255)
                                                                                                 tCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGt                              >  2:596834/1‑139 (MQ=255)
                                                                                                      aCCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCa                         >  2:790642/1‑139 (MQ=255)
                                                                                                         aGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCAtct                      <  1:790642/139‑1 (MQ=255)
                                                                                                           tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaa                                                                                  <  1:813082/77‑1 (MQ=255)
                                                                                                           tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaa                                                                                  >  2:813082/1‑77 (MQ=255)
                                                                                                           tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAg                                                                >  1:1208329/1‑95 (MQ=255)
                                                                                                           tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAg                                                                <  2:1208329/95‑1 (MQ=255)
                                                                                                           tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCa                                                  >  2:534103/1‑109 (MQ=255)
                                                                                                           tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCa                                                  <  1:534103/109‑1 (MQ=255)
                                                                                                           tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATctct                    >  2:336208/1‑139 (MQ=255)
                                                                                                                    tttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAc           >  1:1254199/1‑139 (MQ=255)
                                                                                                                     ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGa                                                                                               <  1:247747/54‑1 (MQ=255)
                                                                                                                     ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGa                                                                                               >  2:247747/1‑54 (MQ=255)
                                                                                                                     ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAAc                                                                        <  2:1056809/77‑1 (MQ=255)
                                                                                                                     ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAAc                                                                        >  1:1056809/1‑77 (MQ=255)
                                                                                                                     ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAcc          >  1:535731/1‑139 (MQ=255)
                                                                                                                     ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAcc          >  1:1500555/1‑139 (MQ=255)
                                                                                                                     ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAcc          >  1:948947/1‑139 (MQ=255)
                                                                                                                     ttCTTGTTGATGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTGCCATCTCTTCCTCCTAcc          >  2:1199720/1‑139 (MQ=255)
                                                                                                                            tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTtcctcc              >  1:945926/1‑128 (MQ=255)
                                                                                                                            tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTtcctcc              <  2:945926/128‑1 (MQ=255)
                                                                                                                             ggTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttct  >  2:1182848/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                  
CTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCT  >  NC_000913/3449992‑3450255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A