Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F1 I2 R1	94	59.5	3027142	91.9%	2781943	108.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,450,125	T→C	T37A (ACC→GCC)	rplV ←	50S ribosomal subunit protein L22

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,450,125	0	T	C	100.0%	198.9 / NA	55	T37A (ACC→GCC)	rplV	50S ribosomal subunit protein L22
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/30); total (25/30)

TGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTC  >  NC_000913/3449990‑3450260

tGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaa                                                                                                                                      <  1:1190963/139‑1 (MQ=255)

 gCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaaa                                                                                                                                     <  2:456565/139‑1 (MQ=255)

 gCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCaaa                                                                                                                                     <  2:909152/139‑1 (MQ=255)

      ggCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCGGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATc                                                                                                                                >  1:98559/1‑139 (MQ=255)

      ggCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTAACCAGTACAGCCGCGTTCTTGTTGGTGTAGGCCAAAATATc                                                                                                                                >  2:1351906/1‑139 (MQ=255)

            cGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGc                                                                                                                          >  1:1235027/1‑139 (MQ=255)

             gTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGcc                                                                                                                         >  2:367644/1‑139 (MQ=255)

              tCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCt                                                                                                                        <  2:68569/139‑1 (MQ=255)

                  cGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGa                                                                                                                    <  2:818614/139‑1 (MQ=255)

                         tttCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTGCAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTc                                                                                                             <  2:1469133/139‑1 (MQ=255)

                                  tttCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGa                                                                                                    >  1:795628/1‑139 (MQ=255)

                                    tCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  <  2:1422756/139‑1 (MQ=255)

                                    tCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAACCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  <  1:657514/139‑1 (MQ=255)

                                       gATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAg                                                                                               <  2:572529/139‑1 (MQ=255)

                                                  tCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGcaacaa                                                                                    <  2:710972/139‑1 (MQ=255)

                                                            cGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTg                                                                                                                       >  1:1418415/1‑94 (MQ=255)

                                                            cGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTg                                                                                                                       <  2:1418415/94‑1 (MQ=255)

                                                                 tgtTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAg                                                                     <  1:1351906/139‑1 (MQ=255)

                                                                     cAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  >  1:414271/1‑106 (MQ=255)

                                                                     cAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAAt                                                                                                  <  2:414271/106‑1 (MQ=255)

                                                                     cAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAAAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCaga                                                                 >  2:924094/1‑139 (MQ=255)

                                                                        cGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCagaaga                                                              >  1:1245985/1‑139 (MQ=255)

                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              <  1:571416/72‑1 (MQ=255)

                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              >  2:571416/1‑72 (MQ=255)

                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               <  2:1296134/119‑1 (MQ=255)

                                                                           tAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               >  1:1296134/1‑119 (MQ=255)

                                                                             gCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCaga                                                                                                                            <  1:58704/72‑1 (MQ=255)

                                                                             gCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCaga                                                                                                                            >  2:58704/1‑72 (MQ=255)

                                                                                    cAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCg                                                  <  1:864473/139‑1 (MQ=255)

                                                                                    cAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCg                                                  <  1:367644/139‑1 (MQ=255)

                                                                                       aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              >  1:34424/1‑60 (MQ=255)

                                                                                       aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCa                                                                                                                              <  2:34424/60‑1 (MQ=255)

                                                                                       aTTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATg                                               >  1:964283/1‑139 (MQ=255)

                                                                                         tCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGtt                                             >  1:73411/1‑139 (MQ=255)

                                                                                                   tCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGt                                   >  2:1438617/1‑139 (MQ=255)

                                                                                                             tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               >  2:1366922/1‑85 (MQ=255)

                                                                                                             tACAGCCGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               <  1:1366922/85‑1 (MQ=255)

                                                                                                                  ccGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         <  2:1103339/102‑1 (MQ=255)

                                                                                                                  ccGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         >  1:1103339/1‑102 (MQ=255)

                                                                                                                  ccGCTTTCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTtcctc                    <  2:878866/139‑1 (MQ=255)

                                                                                                                      tttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAc                <  2:795628/139‑1 (MQ=255)

                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               >  2:538599/1‑75 (MQ=255)

                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGa                                                                               <  1:538599/75‑1 (MQ=255)

                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCt                                                                        >  2:1153533/1‑82 (MQ=255)

                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCt                                                                        <  1:1153533/82‑1 (MQ=255)

                                                                                                                       ttCTTGTTGGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTAcc               >  1:1033100/1‑139 (MQ=255)

                                                                                                                              tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         >  2:1019279/1‑90 (MQ=255)

                                                                                                                              tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttc        <  1:1438617/139‑1 (MQ=255)

                                                                                                                              tgGTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTATTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAg                                                         <  1:1019279/90‑1 (MQ=255)

                                                                                                                               ggTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGc                                                                    >  2:1329186/1‑78 (MQ=255)

                                                                                                                               ggTGTAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGc                                                                    <  1:1329186/78‑1 (MQ=255)

                                                                                                                                gtgtAGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttctt      >  1:1151889/1‑139 (MQ=255)

                                                                                                                                    aGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATg                                               >  2:1431193/1‑94 (MQ=255)

                                                                                                                                    aGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATg                                               <  1:1431193/94‑1 (MQ=255)

                                                                                                                                    aGGCCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATttcttcttc  <  2:1033100/139‑1 (MQ=255)

TGCTCGGGCCTTCGTCTACGAAAATTTTCGTAACTTTCAGATCGTCAATGTCAGCGCCATCGTTGTGTTCAGCGTTAGCAATGGCAGATTCCAGAACTTTCTTGACCAGTACAGCCGCTTTCTTGTTGGTGTAGGTCAAAATATCCAGAGCCTGCGACACTTTCTTACCGCGAATCAGGTCAGCAACAAGGCGAACCTTCTGAGCAGAAGAACGAGCATGGCGATGTTTAGCGATAGTTTCCATCTCTTCCTCCTACCTTATTTCTTCTTC  >  NC_000913/3449990‑3450260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A