Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I2 R1
|
67 |
27.9 |
1239648 |
95.3% |
1181384 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,874,741 |
T→C |
T227A (ACA→GCA) |
cysN ← |
sulfate adenylyltransferase, subunit 1 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,874,741 | 0 | T | C | 100.0%
| 93.1
/ NA
| 25 | T227A (ACA→GCA) | cysN | sulfate adenylyltransferase, subunit 1 |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/10); total (15/10) |
CCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCA > NC_000913/2874611‑2874857
|
ccGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGc > 1:70487/1‑139 (MQ=255)
ccGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATAc < 1:52901/139‑1 (MQ=255)
gTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCa < 2:284798/139‑1 (MQ=255)
aTTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTg < 2:37008/139‑1 (MQ=255)
ggCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGa > 1:396678/1‑92 (MQ=255)
ggCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGa < 2:396678/92‑1 (MQ=255)
aTTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCg < 1:509277/133‑1 (MQ=255)
aTTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCg > 2:509277/1‑133 (MQ=255)
tGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGa > 2:597824/1‑78 (MQ=255)
tGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGa < 1:597824/78‑1 (MQ=255)
gCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCagagag > 2:347455/1‑139 (MQ=255)
cACCTGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGCGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTATCGCCTTCCAGTGCAGAGAGc > 1:358229/1‑139 (MQ=255)
cGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGc > 2:283699/1‑139 (MQ=255)
ggAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcac > 2:464276/1‑139 (MQ=255)
gCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATc < 1:464276/139‑1 (MQ=255)
acTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCa > 2:499581/1‑139 (MQ=255)
acTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCa > 2:25800/1‑139 (MQ=255)
tCGCTGGATCTCCACGGTTTCCAGCACTTCGAGTAGTGCCGGACCGCTGTACCACGGCATAATTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGc > 1:245043/1‑139 (MQ=255)
gCTGGATCTCCACGGTTTCCAGCACTTCGCGCAGTGCCGGACCGCAGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTcc > 1:355590/1‑92 (MQ=255)
gCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTcc < 2:355590/92‑1 (MQ=255)
gCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTcc < 2:453521/92‑1 (MQ=255)
gCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTcc > 1:453521/1‑92 (MQ=255)
tGGATCTCCACGGTTTCCAGCACTTTGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGcc > 2:308594/1‑139 (MQ=255)
cGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGAt < 1:80809/114‑1 (MQ=255)
cGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGAt > 2:80809/1‑114 (MQ=255)
gTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCa > 2:239936/1‑139 (MQ=255)
|
CCGGATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCA > NC_000913/2874611‑2874857
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
