Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	67	31.4	1402038	96.1%	1347358	109.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,874,741	T→C	T227A (ACA→GCA)	cysN ←	sulfate adenylyltransferase, subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,874,741	0	T	C	100.0%	110.0 / NA	29	T227A (ACA→GCA)	cysN	sulfate adenylyltransferase, subunit 1
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/13); total (16/13)

ATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCAAATAATCTTC  >  NC_000913/2874615‑2874867

aTGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTa                                                                                                                    <  2:601345/139‑1 (MQ=255)

      gCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACgg                                                                                                              >  2:175874/1‑139 (MQ=255)

       cGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGc                                                                                                             >  1:271821/1‑139 (MQ=255)

         ttCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCAt                                                                                                           <  1:691714/139‑1 (MQ=255)

          tCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATa                                                                                                          >  1:518220/1‑139 (MQ=255)

                                                     tGCACCGGGAAGCGCTTTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACAACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCagaga                                                               >  1:467146/1‑139 (MQ=255)

                                                     tGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCagaga                                                               >  1:548162/1‑139 (MQ=255)

                                                          cGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGc                                                          >  1:311425/1‑139 (MQ=255)

                                                           gggAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCATGTTGTCGCCTTCCAGTGCAGAGAGCGGca                                                         <  2:467146/139‑1 (MQ=255)

                                                           gggAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGca                                                         <  2:271821/139‑1 (MQ=255)

                                                            ggAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGcac                                                        >  1:485824/1‑139 (MQ=255)

                                                                   cATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCgg                                                 <  2:548162/139‑1 (MQ=255)

                                                                     ttGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGat                                               <  1:175874/139‑1 (MQ=255)

                                                                                      acTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCa                              >  2:647529/1‑139 (MQ=255)

                                                                                        tCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGc                            >  1:2599/1‑139 (MQ=255)

                                                                                                    cACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCa                                                                   >  1:390523/1‑88 (MQ=255)

                                                                                                    cACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCa                                                                   <  2:390523/88‑1 (MQ=255)

                                                                                                    cACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAg                >  1:270313/1‑139 (MQ=255)

                                                                                                    cACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAg                >  2:317102/1‑139 (MQ=255)

                                                                                                    cACGGTTTCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAg                >  2:333596/1‑139 (MQ=255)

                                                                                                          ttCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCaaa          <  2:518220/139‑1 (MQ=255)

                                                                                                           tCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGat                                               >  2:143701/1‑101 (MQ=255)

                                                                                                           tCCAGCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGat                                               <  1:143701/101‑1 (MQ=255)

                                                                                                               gCACTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCAAATAATc     <  1:333596/139‑1 (MQ=255)

                                                                                                                  cTTCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCAAATAATCTTc  <  1:540361/139‑1 (MQ=255)

                                                                                                                   ttCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCaa                  >  1:405537/1‑122 (MQ=255)

                                                                                                                   ttCGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCaa                  <  2:405537/122‑1 (MQ=255)

                                                                                                                     cGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGat                                               >  2:183966/1‑91 (MQ=255)

                                                                                                                     cGAGCAGTGCCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGat                                               <  1:183966/91‑1 (MQ=255)

ATGCCAGCGTTCCGGCGTAACCACGAAAATCGAGATTCGGGCGATTAACGTACTGCACCGGGAAGCGCATTGGCTGAGCATCCACCACTCGCTGGATCTCCACGGTTTCCAGCACTTCGAGCAGTGTCGGACCGCTGTACCACGGCATACTTTCACTTTGCGATGCCACGTTGTCGCCTTCCAGTGCAGAGAGCGGCACAAAGCGGATATCCAGATTACCCGGCAGCTGCCCGGCAAAGGTCAAATAATCTTC  >  NC_000913/2874615‑2874867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A