Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,876,388	C→T	intergenic (‑59/‑193)	cysD ← / → iap	sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,876,388	0	C	T	100.0%	105.1 / NA	32	intergenic (‑59/‑193)	cysD/iap	sulfate adenylyltransferase, subunit 2/aminopeptidase in alkaline phosphatase isozyme conversion
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/16); total (16/16)

TCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAT  >  NC_000913/2876253‑2876512

tCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTa                                                                                                                           >  2:1027174/1‑139 (MQ=255)

  tGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAag                                                                                                                         >  1:581423/1‑139 (MQ=255)

          ccTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGaa                                                                                                                 <  2:581423/139‑1 (MQ=255)

                      tgtgGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCaa                                                                                                     <  1:1027174/139‑1 (MQ=255)

                       gtgGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAAt                                                                                                    <  2:243175/139‑1 (MQ=255)

                           aTGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGa                                                                                                <  2:1239081/139‑1 (MQ=255)

                              cTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATg                                                                                             >  2:1165102/1‑139 (MQ=255)

                                 tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGagt                                                                                          >  2:707285/1‑139 (MQ=255)

                                 tCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGagt                                                                                          <  1:446944/139‑1 (MQ=255)

                                    gCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGagtagt                                                                                       >  2:843165/1‑139 (MQ=255)

                                        ccAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCt                                                                                   >  1:1223074/1‑139 (MQ=255)

                                           gTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTaa                                                                                <  1:578023/139‑1 (MQ=255)

                                                             gTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTATAATGAGTAGTTCCTTAACGGAATAACGATTTGGCa                                                              >  1:746913/1‑139 (MQ=255)

                                                               cGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCaaa                                                            >  1:528848/1‑139 (MQ=255)

                                                                                  ttCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCt                                         >  1:910188/1‑139 (MQ=255)

                                                                                       ttGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAgg                                    >  2:1177778/1‑139 (MQ=255)

                                                                                        tGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCa                                                              >  2:1126011/1‑112 (MQ=255)

                                                                                        tGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCa                                                              <  1:1126011/112‑1 (MQ=255)

                                                                                             tACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTaa                                      <  2:500651/131‑1 (MQ=255)

                                                                                             tACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATAGCAAAAAGTGCTTaa                                      >  1:500651/1‑131 (MQ=255)

                                                                                                     ttttCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCggg                      <  1:707285/139‑1 (MQ=255)

                                                                                                     ttttCTTGCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCggg                      <  2:528848/139‑1 (MQ=255)

                                                                                                            gCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGag                                                                                           <  1:360176/63‑1 (MQ=255)

                                                                                                            gCCATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGag                                                                                           >  2:360176/1‑63 (MQ=255)

                                                                                                              cATCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGa             <  1:1165102/139‑1 (MQ=255)

                                                                                                               aTCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGag                                                                                           <  1:354868/60‑1 (MQ=255)

                                                                                                               aTCAGATGTTTCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGag                                                                                           >  2:354868/1‑60 (MQ=255)

                                                                                                                         tCGACTATAGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAt  <  1:843165/139‑1 (MQ=255)

                                                                                                                               ataGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCg                        <  1:641218/111‑1 (MQ=255)

                                                                                                                               ataGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCg                        >  2:641218/1‑111 (MQ=255)

                                                                                                                                 aGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGagtag                                                                                        >  2:1021690/1‑45 (MQ=255)

                                                                                                                                 aGGGAGTGTAAGAGAACGAATGAAATTACCAATTAGAATGagtag                                                                                        <  1:1021690/45‑1 (MQ=255)

TCTGCCGCCACCTCGCGAATAATGTGGATGCTTTCCGCCTCCAGTTGCCGCAGGTGAGTAAGTCGTATTTGATCCATAACCGTTCCTTTGCAATACCGCTATTTTCTTGCCATCAGATGTTTCGACTATAGGGAGCGTAAGAGAACGAATGAAATTACCAATTAGAATGAGTAGTTCCTTAACGGAATAACGATTTGGCAAAGCTAATATCAAAAAGTGCTTAAGGCACCGGATTTCGGGCGTTTAGGAAGATTTGAAAT  >  NC_000913/2876253‑2876512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A