Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A6 F1 I1 R1
|
67 |
31.4 |
1402038 |
96.1% |
1347358 |
109.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,306,716 |
C→T |
R382H (CGC→CAC) |
deaD ← |
ATP‑dependent RNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,306,716 | 0 | C | T | 100.0%
| 92.6
/ NA
| 28 | R382H (CGC→CAC) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/14); total (14/14) |
CCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGCGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGA > NC_000913/3306603‑3306850
|
ccAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCgg < 1:552761/139‑1 (MQ=255)
cAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGc > 1:609866/1‑139 (MQ=255)
ctTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTc < 1:265408/139‑1 (MQ=255)
ttCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCt < 2:37393/139‑1 (MQ=255)
cACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTAc > 2:213026/1‑139 (MQ=255)
ccTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACtt < 1:213026/139‑1 (MQ=255)
tCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGt > 1:77114/1‑139 (MQ=255)
gCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAg < 2:609866/139‑1 (MQ=255)
tGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGt < 2:77114/139‑1 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGt > 1:286877/1‑100 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGt < 2:286877/100‑1 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTgcgc > 2:78505/1‑128 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTgcgc < 1:78505/128‑1 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCgcag < 2:699710/130‑1 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCgcag > 1:699710/1‑130 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGacgacg > 1:293157/1‑139 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGacgacg > 2:512216/1‑139 (MQ=255)
gATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGacgacg > 2:493093/1‑139 (MQ=255)
tgctTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGATGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAgac < 1:66331/129‑1 (MQ=255)
tgctTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAgac > 2:66331/1‑129 (MQ=255)
tCCAGCTGCTGCTGTACTTTAGCGGCGAA‑TTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACg < 2:644106/103‑1 (MQ=255)
tCCAGCTGCTGCTGTACTTTAGCGGCGAA‑TTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACg > 1:644106/1‑103 (MQ=255)
cAGCTGCTCCTGTACTTTAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCa > 2:625919/1‑139 (MQ=255)
tAGCGGCGAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGcgcgcg > 1:491906/1‑139 (MQ=255)
cggcgAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTAc > 1:193602/1‑55 (MQ=255)
cggcgAATTTTTCCAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTAc < 2:193602/55‑1 (MQ=255)
cAGACGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCa < 2:293157/139‑1 (MQ=255)
aCGGTGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGa < 2:491906/139‑1 (MQ=255)
|
CCAGCTCTTCACCTTCAGCAGTCGGCTGAATTTTGCTCAGCAGTGCGCGGTATTGATCCAGATCGCTGCTTTCCAGCTGCTGCTGTACTTTAGCGGCGAATTTTTCCAGACGGCGTTTGCCTAGCAGTTCTGCGTTCGGCAGTTCTACTTCCGGAATAGTCAGCTTCATAGTACGTTCAATGTTGCGCAGCAGACGACGCTCGCGGTTCTCAACGAACAGCAGCGCGCGGCCAGCACGACCCGCACGA > NC_000913/3306603‑3306850
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
