Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F1 I1 R1	67	31.4	1402038	96.1%	1347358	109.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,490,935	A→G	L69L (TTG→CTG)	idnR ←	transcriptional repressor, 5‑gluconate‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,490,935	0	A	G	100.0%	187.7 / NA	50	L69L (TTG→CTG)	idnR	transcriptional repressor, 5‑gluconate‑binding
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/28); total (22/28)

ATCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCG  >  NC_000913/4490802‑4491061

aTCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAc                                                                                                                           >  1:142528/1‑139 (MQ=255)

 tCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATAcc                                                                                                                          <  2:142528/139‑1 (MQ=255)

  cACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCg                                                                                                                         <  2:593121/139‑1 (MQ=255)

      gACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAggg                                                                                                                     >  1:65013/1‑139 (MQ=255)

           ttcttcGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATaa                                                                                                                >  2:636120/1‑139 (MQ=255)

                  gAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTtgtg                                                                                                         <  2:53387/139‑1 (MQ=255)

                   aaTCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGc                                                                                                        >  1:613136/1‑139 (MQ=255)

                     tCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCAt                                                                                                      >  1:50525/1‑139 (MQ=255)

                          aTCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaac                                                                                                 <  1:438711/139‑1 (MQ=255)

                                      attAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                        <  2:525506/104‑1 (MQ=255)

                                      attAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                        >  1:525506/1‑104 (MQ=255)

                                      attAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGa                                                                                     >  1:689452/1‑139 (MQ=255)

                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                        <  2:7429/103‑1 (MQ=255)

                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                        >  1:7429/1‑103 (MQ=255)

                                       ttAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAg                                                                                    >  1:221624/1‑139 (MQ=255)

                                         aGCAATAAGGGTCTGATAATTATGCTCTGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAAGATGCCGGGAgcg                                                                                  >  1:417089/1‑139 (MQ=255)

                                         aGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAgcg                                                                                  >  1:309162/1‑139 (MQ=255)

                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                             <  2:309162/139‑1 (MQ=255)

                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                             <  2:689452/139‑1 (MQ=255)

                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                             <  2:65013/139‑1 (MQ=255)

                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAtt                                                                             <  2:285937/139‑1 (MQ=255)

                                              tAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATACAACAACATGCCGGGATCGCGAtt                                                                             <  2:426564/139‑1 (MQ=255)

                                                   gTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                        <  2:613136/139‑1 (MQ=255)

                                                     cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTTAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGata                                                                      <  2:417089/139‑1 (MQ=255)

                                                     cTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGata                                                                      <  2:29171/139‑1 (MQ=255)

                                                             tATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                              <  2:552718/139‑1 (MQ=255)

                                                                    agagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTcc                                                       >  2:135388/1‑139 (MQ=255)

                                                                     gagGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCa                                                      <  2:477928/139‑1 (MQ=255)

                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                        <  1:366011/72‑1 (MQ=255)

                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGa                                                                                                                        >  2:366011/1‑72 (MQ=255)

                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                              <  1:253337/130‑1 (MQ=255)

                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                              >  2:253337/1‑130 (MQ=255)

                                                                      agGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCAt                                                     >  2:532575/1‑139 (MQ=255)

                                                                         tGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                        >  1:186801/1‑117 (MQ=255)

                                                                         tGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                        <  2:186801/117‑1 (MQ=255)

                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAt                                                                              <  1:119973/109‑1 (MQ=255)

                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGAt                                                                              >  2:119973/1‑109 (MQ=255)

                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAAttt                                                >  2:48851/1‑139 (MQ=255)

                                                                           aCGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGCGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAAttt                                                >  2:434729/1‑139 (MQ=255)

                                                                                         cgcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                              <  1:199217/111‑1 (MQ=255)

                                                                                         cgcgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGAt                                                              >  2:199217/1‑111 (MQ=255)

                                                                                           cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaacaa                                                                                               >  2:571043/1‑76 (MQ=255)

                                                                                           cgAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTcaacaa                                                                                               <  1:571043/76‑1 (MQ=255)

                                                                                               aaTGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                        >  2:178458/1‑95 (MQ=255)

                                                                                               aaTGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGa                                                                        <  1:178458/95‑1 (MQ=255)

                                                                                                                  ttttGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCg         <  1:237718/139‑1 (MQ=255)

                                                                                                                   tttGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGTGCTCGCCTGTTTCCTTTGCCACCTTTTTCgg        <  1:636120/139‑1 (MQ=255)

                                                                                                                     tGAAATGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCGGTTTCCGTTGCCACCATTTTCGGCg      <  1:406606/139‑1 (MQ=255)

                                                                                                                       aaaTGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAg    <  1:48851/139‑1 (MQ=255)

                                                                                                                         aTGACGGAATCAGGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCg  <  2:221624/139‑1 (MQ=255)

ATCACCGACTCTTCTTCGGAATCGCGATCGTAATTGTAATTAGCAATAAGGGTCTGATAATTATGCTCAGAGGTGACGGATTCTATTCCCGCGAGAATGTCAGCGAAGAGTTGATTTTGAAATGACGGAATCAAGATACCGAGGGTATAACTTTGTGCATTCAACAACATGCCGGGAGCGCGATTAGGGATATAATTGATCTCTTCCATAATTTTGGCGATGCGCTCGCCTGTTTCCTTTGCCACCTTTTTCGGCGAGCG  >  NC_000913/4490802‑4491061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A