Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I1 R1	63	64.8	2934156	93.7%	2749304	111.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,268,372	G→A	E182K (GAG→AAG)	yeiR →	Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,268,372	0	G	A	100.0%	153.1 / NA	44	E182K (GAG→AAG)	yeiR	Zn‑stimulated GTPase involved in zinc homeostasis, mutants are cadmium and EDTA sensitive, Zn(2+) binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/24); total (20/24)

AAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAG  >  NC_000913/2268238‑2268498

aaaaCTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCa                                                                                                                            <  2:475546/139‑1 (MQ=255)

              cAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACTGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACg                                                                                                              <  1:1189283/139‑1 (MQ=255)

              cAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACg                                                                                                              >  1:595069/1‑139 (MQ=255)

               aGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACgg                                                                                                             >  2:21800/1‑139 (MQ=255)

                      tgCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCAtctt                                                                                                      >  1:759551/1‑139 (MQ=255)

                            aGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGAt                                                                                                <  1:1109877/139‑1 (MQ=255)

                                atcatTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGc                                                                                            <  1:191862/139‑1 (MQ=255)

                                atcatTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGc                                                                                            <  2:764376/139‑1 (MQ=255)

                                     tGTCGCCAATAAATCCGACCGTCCGACGCCCGAAAGTGAGCAAGAGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGt                                                                                       >  1:1396922/1‑139 (MQ=255)

                                     tGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGt                                                                                       <  1:592962/139‑1 (MQ=255)

                                            aaTAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAAtt                                                                                >  1:1351194/1‑139 (MQ=255)

                                                      aCCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTc                                                                                                           <  1:578728/102‑1 (MQ=255)

                                                      aCCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTc                                                                                                           >  2:578728/1‑102 (MQ=255)

                                                          tACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGccc                                                                  >  2:686795/1‑139 (MQ=255)

                                                          tACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGccc                                                                  >  1:563370/1‑139 (MQ=255)

                                                            cgacgCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGc                                                                >  1:1354987/1‑139 (MQ=255)

                                                                     aaaGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGcgccgc                                                       <  2:1351194/139‑1 (MQ=255)

                                                                     aaaGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGcgccgc                                                       >  1:1103970/1‑139 (MQ=255)

                                                                        gTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa                                                    <  2:81500/139‑1 (MQ=255)

                                                                         tGAGCGAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGTAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt                                                   <  1:911006/139‑1 (MQ=255)

                                                                            gCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATtct                                                <  1:211062/139‑1 (MQ=255)

                                                                              aaGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCtca                                              <  2:1396922/139‑1 (MQ=255)

                                                                                     aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCcg                                                                                          >  1:1033181/1‑88 (MQ=255)

                                                                                     aCAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCcg                                                                                          <  2:1033181/88‑1 (MQ=255)

                                                                                            tggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAtt                                                  >  1:371710/1‑121 (MQ=255)

                                                                                            tggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAtt                                                  <  2:371710/121‑1 (MQ=255)

                                                                                            tggtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGa                                >  1:1019793/1‑139 (MQ=255)

                                                                                              gtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGt                                                                                                            >  2:741687/1‑61 (MQ=255)

                                                                                              gtggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGt                                                                                                            <  1:741687/61‑1 (MQ=255)

                                                                                                ggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaaa                            <  2:687248/139‑1 (MQ=255)

                                                                                                ggCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGaaaaa                            <  2:300109/139‑1 (MQ=255)

                                                                                                   agcaAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa                                                    <  2:230316/112‑1 (MQ=255)

                                                                                                   agcaAAATGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCa                                                    >  1:230316/1‑112 (MQ=255)

                                                                                                        aaTGGTGGCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTag                    <  2:803443/139‑1 (MQ=255)

                                                                                                               gCGATCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGtt             >  2:1399145/1‑139 (MQ=255)

                                                                                                                  aTCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTg                                                                     <  2:878307/80‑1 (MQ=255)

                                                                                                                  aTCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTg                                                                     >  1:878307/1‑80 (MQ=255)

                                                                                                                   tCGACAATTAATTCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGc         <  1:686795/139‑1 (MQ=255)

                                                                                                                         attaattCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCa   >  1:65315/1‑139 (MQ=255)

                                                                                                                          ttaattCACAGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCag  <  2:759551/139‑1 (MQ=255)

                                                                                                                                  caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCg                                                                          >  2:1390543/1‑59 (MQ=255)

                                                                                                                                  caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCg                                                                          <  1:1390543/59‑1 (MQ=255)

                                                                                                                                  caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt                                                   >  2:246918/1‑82 (MQ=255)

                                                                                                                                  caGTAAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCAt                                                   <  1:246918/82‑1 (MQ=255)

AAAACTTCCGTGACCAGCTGGCTGCCGCAGACATCATTGTCGCCAATAAATCCGACCGTACGACGCCCGAAAGTGAGCAAGCGCTACAGCGTTGGTGGCAGCAAAATGGTGGCGATCGACAATTAATTCACAGTGAGCATGGGAAAGTTGACGGTCATCTTCTGGATTTGCCGCGTCGCAATTTAGCCGAGTTGCCCGCCAGCGCCGCGCATTCTCATCAGCATGTCGTGAAAAAAGGGTTAGCAGCGTTAAGCCTGCCAG  >  NC_000913/2268238‑2268498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A