Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,277,291	C→T	A331V (GCG→GTG)	yejF →	microcin C ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,277,291	0	C	T	100.0%	145.0 / NA	42	A331V (GCG→GTG)	yejF	microcin C ABC transporter ATPase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (21/21); total (21/21)

CATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAA  >  NC_000913/2277157‑2277413

cATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTgctg                                                                                                                        >  2:702000/1‑139 (MQ=255)

 aTTCGCAAAGGGCTCTTGAAGCGCCGTGTGGATCATAATTTGGTGGTGAGAAGCATCAGTTTTACGCTACGAGCGGCTGAAACGGTGGGTTTCGAGGGCAAGTCCGGTTCCGGGACAACTACGACGGGAGTGGTgctgt                                                                                                                       >  1:489999/1‑138 (MQ=255)

   tcacAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGctgctg                                                                                                                     >  2:816653/4‑139 (MQ=255)

   tCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGctgctg                                                                                                                     >  2:374775/1‑139 (MQ=255)

      cAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGa                                                                                                                  <  1:935036/139‑1 (MQ=255)

      cAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGa                                                                                                                  <  2:2584/139‑1 (MQ=255)

        aaGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACt                                                                                                                <  2:789524/139‑1 (MQ=255)

           ggATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGAt                                                                                                             <  1:816653/139‑1 (MQ=255)

                     gcgcATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGG‑AAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAgg                                                                                                  >  2:68088/1‑139 (MQ=255)

                       gcATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAggg                                                                                                 <  1:94175/139‑1 (MQ=255)

                                     aaTGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTg                                                                                   >  2:596139/1‑139 (MQ=255)

                                           gtggtgAAAAACATCAGTTTTACGCTACGAGTGGGTGAAACAGTGGGTTTAGTGGGCGAGTCTGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATGCTCAGGGCAGCATCATCTTTGACGGTc                                                                             >  1:82761/1‑139 (MQ=255)

                                           gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTc                                                                             >  1:824863/1‑139 (MQ=255)

                                            tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  1:43899/1‑139 (MQ=255)

                                            tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  2:850654/1‑139 (MQ=255)

                                            tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  2:981654/1‑139 (MQ=255)

                                            tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCa                                                                            >  1:270360/1‑139 (MQ=255)

                                             ggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAg                                                                           >  2:999743/1‑139 (MQ=255)

                                              gtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAGGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGc                                                                          <  1:654649/139‑1 (MQ=255)

                                                                     aCGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccgc                                                   <  1:293207/139‑1 (MQ=255)

                                                                     aCGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGG‑AAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCgccgcc                                                  <  1:68088/139‑1 (MQ=255)

                                                                      cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTgctg                                                                                                                        <  2:508160/69‑1 (MQ=255)

                                                                      cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTgctg                                                                                                                        >  1:508160/1‑69 (MQ=255)

                                                                      cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCgccgcc                                                  <  1:999743/139‑1 (MQ=255)

                                                                          cGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCt                                              <  2:43899/139‑1 (MQ=255)

                                                                             gTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGtt                                           <  2:270360/139‑1 (MQ=255)

                                                                              tGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTa                                          >  1:445417/1‑139 (MQ=255)

                                                                                        ggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATGGCCGCCAGCTGTTACCTATTCGTc                                >  2:619600/1‑139 (MQ=255)

                                                                                        ggTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTc                                >  2:805449/1‑139 (MQ=255)

                                                                                         gTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCa                               <  1:981654/139‑1 (MQ=255)

                                                                                         gTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCa                               <  1:374775/139‑1 (MQ=255)

                                                                                          tttAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCAt                              <  2:82761/139‑1 (MQ=255)

                                                                                                gggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCAtt                        <  1:932461/139‑1 (MQ=255)

                                                                                                  gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa                      >  2:65475/1‑139 (MQ=255)

                                                                                                  gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa                      >  2:588572/1‑139 (MQ=255)

                                                                                                    gAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAgg                    <  1:805449/139‑1 (MQ=255)

                                                                                                    gAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAgg                    <  2:824863/139‑1 (MQ=255)

                                                                                                     aGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAggt                   <  1:588572/139‑1 (MQ=255)

                                                                                                             tCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTc           <  2:77201/139‑1 (MQ=255)

                                                                                                                gggAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAgg        >  2:578787/1‑139 (MQ=255)

                                                                                                                gggAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAg         <  2:21066/138‑1 (MQ=255)

                                                                                                                gggAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAg         >  1:21066/1‑138 (MQ=255)

                                                                                                                      aGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCaa  >  1:354279/1‑139 (MQ=255)

CATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATTTCAGGATCCAA  >  NC_000913/2277157‑2277413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A