Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
77 |
34.8 |
1608548 |
95.0% |
1528120 |
114.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,277,291 |
C→T |
A331V (GCG→GTG) |
yejF → |
microcin C ABC transporter ATPase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,277,291 | 0 | C | T | 100.0%
| 81.5
/ NA
| 25 | A331V (GCG→GTG) | yejF | microcin C ABC transporter ATPase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (15/10); total (15/10) |
TCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATT > NC_000913/2277160‑2277402
|
tCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGctgctg > 1:387862/1‑139 (MQ=255)
aGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGATTTCCGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTg > 1:50024/1‑139 (MQ=255)
gAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATtct < 2:50024/139‑1 (MQ=255)
gcgcATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGGTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAg > 1:256971/1‑139 (MQ=255)
cATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgc > 2:93292/1‑139 (MQ=255)
tgtgGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGtgctgc < 1:264604/101‑1 (MQ=255)
tgtgGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGtgctgc > 2:264604/1‑101 (MQ=255)
tgtgGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGAc > 1:238940/1‑139 (MQ=255)
gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa > 2:20954/1‑134 (MQ=255)
gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGa < 1:20954/134‑1 (MQ=255)
gtggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTc > 1:699153/1‑139 (MQ=255)
tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcagca > 1:455518/1‑123 (MQ=255)
tggtgAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcagca < 2:455518/123‑1 (MQ=255)
gTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAAttt > 2:413344/1‑139 (MQ=255)
cGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcg > 1:271745/1‑139 (MQ=255)
aCGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATcgccgc < 1:215949/139‑1 (MQ=255)
cGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTTAGTGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCgccgcc > 1:319787/1‑139 (MQ=255)
aGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAg < 2:52108/139‑1 (MQ=255)
ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcag < 1:304308/89‑1 (MQ=255)
ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGgcag > 2:304308/1‑89 (MQ=255)
ggTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCGACTGCAAAATTTAAATCGCCGCCAGCTGt > 1:516935/1‑139 (MQ=255)
gTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGtt < 2:238940/139‑1 (MQ=255)
tttAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCAt < 2:699153/139‑1 (MQ=255)
gggCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCAtt < 2:387862/139‑1 (MQ=255)
gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa < 1:667331/139‑1 (MQ=255)
gCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCa > 1:7132/1‑139 (MQ=255)
gTTCCGGGAAAAGTACGACGGGACTGGTGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTAtt > 2:301880/1‑139 (MQ=255)
|
TCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAAGTACGACGGGACTGGCGCTGCTGCGACTGATTAATTCTCAGGGCAGCATCATCTTTGACGGTCAGCCACTGCAAAATTTAAATCGCCGCCAGCTGTTACCTATTCGTCATCGCATTCAGGTGGTATT > NC_000913/2277160‑2277402
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
