Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I2 R1	70	59.9	2639336	94.5%	2494172	113.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,035,437	T→C	K282R (AAG→AGG)	prfB ←	peptide chain release factor RF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,035,437	0	T	C	100.0%	199.4 / NA	54	K282R (AAG→AGG)	prfB	peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/29); total (25/29)

TAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGAT  >  NC_000913/3035305‑3035569

tAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCt                                                                                                                                >  1:1200146/1‑139 (MQ=255)

tAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCt                                                                                                                                >  2:256852/1‑139 (MQ=255)

   gAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTggg                                                                                                                             >  1:344760/1‑139 (MQ=255)

       gAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACg                                                                                                                         >  1:915580/1‑139 (MQ=255)

         aTCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGt                                                                                                                       >  1:259954/1‑139 (MQ=255)

             ggCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGtt                                                                                                                   <  2:259954/139‑1 (MQ=255)

               cTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCt                                                                                                                 >  2:908484/1‑139 (MQ=255)

                   cccAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                             <  1:440806/139‑1 (MQ=255)

                           aTGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCa                                                                                                     <  1:304577/139‑1 (MQ=255)

                            tGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCAc                                                                                                    >  2:542371/1‑139 (MQ=255)

                            tGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCAc                                                                                                    >  1:427349/1‑139 (MQ=255)

                                 gATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATcc                                                                                               <  2:915580/139‑1 (MQ=255)

                                   tttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCg                                                                                             <  1:256852/139‑1 (MQ=255)

                                    ttGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTTCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGGACGGTCGTTCTGGCACTGGGGGACGATCCCgg                                                                                            <  1:904478/139‑1 (MQ=255)

                                     tGTTATCTTCCATCGCCTGTTTCTCGTCATTTTTCTTCTGCATCTCCAATTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                           <  1:1155004/139‑1 (MQ=255)

                                        tATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCgg                                                                                        <  2:25093/139‑1 (MQ=255)

                                        tATCTTCCATCGCCTGTCTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTCGCACGGGGTCACGATCCAGGTCgg                                                                                        >  1:1175457/1‑139 (MQ=255)

                                               cATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                 <  1:748337/139‑1 (MQ=255)

                                               cATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTgg                                                                                 <  1:1066385/139‑1 (MQ=255)

                                                    ccTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                           >  2:739111/1‑124 (MQ=255)

                                                    ccTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGt                                                                                           <  1:739111/124‑1 (MQ=255)

                                                                     ttcttcTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCgg                                                           >  1:1213798/1‑139 (MQ=255)

                                                                        ttctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTAc                                                        <  2:1200146/139‑1 (MQ=255)

                                                                          ctGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACgg                                                      <  2:344760/139‑1 (MQ=255)

                                                                            gCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGtt                                                    <  2:427349/139‑1 (MQ=255)

                                                                                           aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCggg                                                                                       >  1:316951/1‑89 (MQ=255)

                                                                                           aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCggg                                                                                       <  2:316951/89‑1 (MQ=255)

                                                                                           aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     >  1:556789/1‑139 (MQ=255)

                                                                                           aaaGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     <  2:1175457/139‑1 (MQ=255)

                                                                                                 tCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGcc                               >  2:916131/1‑139 (MQ=255)

                                                                                                  cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACt                                                                                                           >  2:167612/1‑62 (MQ=255)

                                                                                                  cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACt                                                                                                           <  1:167612/62‑1 (MQ=255)

                                                                                                  cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                              >  1:580143/1‑139 (MQ=255)

                                                                                                  cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                              >  2:486839/1‑139 (MQ=255)

                                                                                                  cGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCg                              >  1:305726/1‑139 (MQ=255)

                                                                                                   gCTTTCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCgg                             >  1:1206293/1‑139 (MQ=255)

                                                                                                      ttCATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACg                          <  2:556789/139‑1 (MQ=255)

                                                                                                        cATCTGCTTCATGGCCTGATTTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGAATGTGGGTAATACGCACCGAAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTg                        <  2:580143/139‑1 (MQ=255)

                                                                                                        cATCTGCTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTg                        <  1:106866/139‑1 (MQ=255)

                                                                                                              cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     <  1:216733/120‑1 (MQ=255)

                                                                                                              cTTCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGAccgcc                                     >  2:216733/1‑120 (MQ=255)

                                                                                                               ttCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                 >  1:469603/1‑139 (MQ=255)

                                                                                                               ttCATGGCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATaaa                 <  2:729398/139‑1 (MQ=255)

                                                                                                                    ggCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCa            >  2:1019484/1‑139 (MQ=255)

                                                                                                                    ggCCTGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCa            <  2:690030/139‑1 (MQ=255)

                                                                                                                        tGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAt                                                                                     >  1:637817/1‑62 (MQ=255)

                                                                                                                        tGATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGAt                                                                                     <  2:637817/62‑1 (MQ=255)

                                                                                                                         gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                             >  1:1148856/1‑37 (MQ=255)

                                                                                                                         gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCa                                                                                                             <  2:1148856/37‑1 (MQ=255)

                                                                                                                         gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                           <  2:33730/119‑1 (MQ=255)

                                                                                                                         gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGAc                           >  1:33730/1‑119 (MQ=255)

                                                                                                                         gATCTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATgcg       <  1:916131/139‑1 (MQ=255)

                                                                                                                            cTTTGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAg    <  1:1099520/139‑1 (MQ=255)

                                                                                                                              ttGTTCCTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTACTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGAt  <  1:873735/139‑1 (MQ=255)

TAAGAACGAATCTGGCTGCCCCAGCCGATGTCGGATTTGTTATCTTCCATCGCCTGTTTCTCGGCATTTTTCTTCTGCATCTCCAGTTCATAAAGCTTCGCTTTCATCTGCTTCATGGCCTGATCTTTGTTCTTGTGCTGGGAACGGTCGTTCTGGCACTGGGTCACGATCCCGGTCGGGATGTGGGTAATACGCACCGCAGATTCGGTACGGTTAACGTGCTGACCGCCCGCGCCGGACGTGCGATAAACGTCAATGCGCAGAT  >  NC_000913/3035305‑3035569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A