Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
94 |
59.5 |
3027142 |
91.9% |
2781943 |
108.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,225,294 |
A→G |
G489G (GGA→GGG) |
metH → |
homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,225,294 | 0 | A | G | 100.0%
| 205.5
/ NA
| 56 | G489G (GGA→GGG) | metH | homocysteine‑N5‑methyltetrahydrofolate transmethylase, B12‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/25); total (31/25) |
TGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAG > NC_000913/4225161‑4225427
|
tGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAg < 1:1117180/137‑1 (MQ=255)
tGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAg > 2:1117180/1‑137 (MQ=255)
ggCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCAc < 2:21620/139‑1 (MQ=255)
aGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGaaa > 1:1347280/1‑139 (MQ=255)
aGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGaaa > 1:1483764/1‑139 (MQ=255)
ttGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCga > 2:1094790/1‑139 (MQ=255)
tGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCgag > 2:778704/1‑139 (MQ=255)
tctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCg > 1:1233940/1‑139 (MQ=255)
ctcGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCgg > 2:622151/1‑139 (MQ=255)
cGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 1:778704/139‑1 (MQ=255)
cGATAAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 2:439027/139‑1 (MQ=255)
aTGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGt > 1:820561/1‑139 (MQ=255)
gAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTAc < 2:820561/139‑1 (MQ=255)
agagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTACTGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa > 2:178994/1‑139 (MQ=255)
agagGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACaaa > 1:794431/1‑139 (MQ=255)
cGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTc > 1:1244073/1‑139 (MQ=255)
gTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 1:169853/139‑1 (MQ=255)
aTGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGa < 2:1483764/139‑1 (MQ=255)
cTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAagag > 1:577479/1‑139 (MQ=255)
tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa > 1:390538/1‑128 (MQ=255)
tATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCa < 2:390538/128‑1 (MQ=255)
tCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTc > 2:1456370/1‑139 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCggg > 2:777542/1‑106 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCggg < 1:777542/106‑1 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTAc < 2:45941/111‑1 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTAc > 1:45941/1‑111 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAAt < 1:1335541/116‑1 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAAt > 2:1335541/1‑116 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc > 1:467785/1‑139 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc < 2:794431/139‑1 (MQ=255)
cACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTcc < 2:1237495/139‑1 (MQ=255)
aaaTTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCag < 2:577479/139‑1 (MQ=255)
tCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc > 1:1335167/1‑118 (MQ=255)
tCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGc < 2:1335167/118‑1 (MQ=255)
tCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATatcatc > 1:1381566/1‑139 (MQ=255)
gCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCtt < 1:1193274/139‑1 (MQ=255)
gCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCtt > 2:1193274/1‑139 (MQ=255)
tGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt > 2:721696/1‑76 (MQ=255)
tGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt < 1:721696/76‑1 (MQ=255)
gtAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGATCCAAACATCTTCGCgg < 1:781382/139‑1 (MQ=255)
tAATGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGt < 2:836813/139‑1 (MQ=255)
tGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTcg > 1:210654/1‑132 (MQ=255)
tGGCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTcg < 2:210654/132‑1 (MQ=255)
ggCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCtt > 1:459223/1‑93 (MQ=255)
ggCCTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCtt < 2:459223/93‑1 (MQ=255)
ccTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAAc < 2:1233940/139‑1 (MQ=255)
cTTTGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACt > 1:1078998/1‑139 (MQ=255)
ttGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt < 2:187512/53‑1 (MQ=255)
ttGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGt > 1:187512/1‑53 (MQ=255)
ttGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTgg > 1:206001/1‑139 (MQ=255)
tGACGAACAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc > 1:1059880/1‑139 (MQ=255)
tGACGAACAGGGGCAGGCCGATACTCGCCCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCACACCGAAGAGAGTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGc > 2:301675/1‑139 (MQ=255)
aCAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGaa > 1:78073/1‑139 (MQ=255)
cAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGc < 1:897799/50‑1 (MQ=255)
cAGGGGCAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTTACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAag < 2:1078998/139‑1 (MQ=255)
cAGGGGCAGGCCGATACTCGCGCACGGAAAATAGAGATTTGCCGTCGGGc > 2:897799/1‑50 (MQ=255)
|
TGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAG > NC_000913/4225161‑4225427
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
