Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I2 R1
|
94 |
59.5 |
3027142 |
91.9% |
2781943 |
108.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,341,750 |
G→A |
intergenic (‑122/‑161) |
melR ← / → melA |
melibiose operon transcriptional regulator, autoregulator/alpha‑galactosidase, NAD(P)‑binding |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,341,750 | 0 | G | A | 98.3%
| 198.8
/ NA
| 59 | intergenic (‑122/‑161) | melR/melA | melibiose operon transcriptional regulator, autoregulator/alpha‑galactosidase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (33/25); minor base T (0/1); total (33/26) |
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAG > NC_000913/4341625‑4341884
|
tCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGc > 1:142261/1‑139 (MQ=255)
tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 2:992406/1‑139 (MQ=255)
tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 2:1511381/1‑139 (MQ=255)
tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 1:151143/1‑139 (MQ=255)
tggCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGGGAGGTAGGCTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCATAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAg > 2:295218/1‑139 (MQ=255)
gCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGt > 2:103491/1‑139 (MQ=255)
gCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGt > 1:1405998/1‑139 (MQ=255)
ctctTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAAt > 1:655977/1‑139 (MQ=255)
tCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAtt < 2:151143/139‑1 (MQ=255)
tGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTccc < 2:1198040/139‑1 (MQ=255)
tCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAtt < 2:1438870/129‑1 (MQ=255)
tCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAtt > 1:1438870/1‑129 (MQ=255)
tCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACt < 1:103491/139‑1 (MQ=255)
tGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTAc > 2:749773/1‑139 (MQ=255)
ggAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCTAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActg < 1:1086936/136‑1 (MQ=255)
ggAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCTAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActg > 2:1086936/1‑136 (MQ=255)
aGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTgctgct > 1:636157/1‑139 (MQ=255)
ggaggaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGc < 1:295218/139‑1 (MQ=255)
gaTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGa > 2:228800/1‑139 (MQ=255)
aTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGAt < 2:655977/139‑1 (MQ=255)
aTGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGAt < 2:1425445/139‑1 (MQ=255)
aCTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTg > 1:377873/1‑139 (MQ=255)
cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACg > 2:1160132/1‑126 (MQ=255)
cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACg < 1:1160132/126‑1 (MQ=255)
cGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGtt > 1:141472/1‑139 (MQ=255)
aGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc < 2:1073474/104‑1 (MQ=255)
aGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc > 1:1073474/1‑104 (MQ=255)
gggAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATgg < 1:1511381/139‑1 (MQ=255)
cGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAA‑‑CTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCa > 1:1375709/1‑139 (MQ=255)
ttttCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCaa > 1:600949/1‑139 (MQ=255)
cccTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGa > 2:357985/1‑139 (MQ=255)
ctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCttt < 2:1122514/56‑1 (MQ=255)
ctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCttt > 1:1122514/1‑56 (MQ=255)
ctTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGcc > 1:1091150/1‑139 (MQ=255)
cAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGACCGAAAA‑‑CTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGtt < 2:1375709/139‑1 (MQ=255)
ggggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCA‑GATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCgg < 1:599504/131‑1 (MQ=255)
ggggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCA‑GATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCgg > 2:599504/1‑131 (MQ=255)
ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCa < 1:1037509/63‑1 (MQ=255)
ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCa > 2:1037509/1‑63 (MQ=255)
ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActgct > 2:756832/1‑82 (MQ=255)
ggCGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTActgct < 1:756832/82‑1 (MQ=255)
cGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGc > 1:1130010/1‑139 (MQ=255)
cGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGc > 2:311804/1‑139 (MQ=255)
cGAGGGGACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTATGc > 1:298319/1‑139 (MQ=255)
ggggACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGa < 2:1405998/139‑1 (MQ=255)
ggggACTCTCCGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGa < 1:357985/139‑1 (MQ=255)
ccGAGTATCATGAGTCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGAGCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGc < 2:298319/139‑1 (MQ=255)
ccGAGTATCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGc < 2:1091150/139‑1 (MQ=255)
tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAt > 1:355731/1‑37 (MQ=255)
tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTAt < 2:355731/37‑1 (MQ=255)
tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc < 2:1391923/49‑1 (MQ=255)
tCATGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTc > 1:1391923/1‑49 (MQ=255)
aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCa < 1:438594/69‑1 (MQ=255)
aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCa > 2:438594/1‑69 (MQ=255)
aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGaa > 1:285789/1‑139 (MQ=255)
aTGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGaa < 2:600949/139‑1 (MQ=255)
tGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCt > 2:280318/1‑74 (MQ=255)
tGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCt < 1:280318/74‑1 (MQ=255)
tGAGACCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAg < 2:142261/139‑1 (MQ=255)
|
TCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTCTGCTTTTCAGGTAATTTATTCCCATAAACTCAGATTTACTGCTGCTTCACGCAGGATCTGAGTTTATGGGAATGCTCAACCTGGAAGCCGGAGGTTTTCTGCAGATTCGCCTGCCATGATGAAG > NC_000913/4341625‑4341884
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
