Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	77	35.1	1569012	96.6%	1515665	112.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,541	A→T	I58F (ATC→TTC)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,541	0	A	T	100.0%	128.2 / NA	38	I58F (ATC→TTC)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (18/20); total (18/20)

CAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAG  >  NC_000913/1806430‑1806675

cAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCg                                                                                                             >  1:583387/1‑139 (MQ=255)

             aaGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTc                                                                                                >  2:347610/1‑139 (MQ=255)

              aGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               <  2:125240/139‑1 (MQ=255)

                                  cACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               >  1:228654/1‑119 (MQ=255)

                                  cACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               <  2:228654/119‑1 (MQ=255)

                                  cACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGt                                                                           >  2:505735/1‑139 (MQ=255)

                                     cGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTccc                                                                        >  1:580451/1‑139 (MQ=255)

                                       gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  2:411966/1‑139 (MQ=255)

                                       gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  2:327458/1‑139 (MQ=255)

                                       gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  1:506080/1‑139 (MQ=255)

                                       gtgtTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg                                                                      >  1:64502/1‑139 (MQ=255)

                                                  cGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGaa                                                                                                           <  2:703562/91‑1 (MQ=255)

                                                  cGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGaa                                                                                                           >  1:703562/1‑91 (MQ=255)

                                                   gggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAg                                                          <  2:5547/139‑1 (MQ=255)

                                                    ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCg                                                                                                             >  2:601109/1‑87 (MQ=255)

                                                    ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCg                                                                                                             <  1:601109/87‑1 (MQ=255)

                                                    ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     <  1:134406/127‑1 (MQ=255)

                                                    ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     >  2:134406/1‑127 (MQ=255)

                                                    ggggCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGa                                                         >  2:718820/1‑139 (MQ=255)

                                                         ggcggcATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCa                                                    <  2:506080/139‑1 (MQ=255)

                                                         ggcggcATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCa                                                    >  2:379191/1‑139 (MQ=255)

                                                                   aCGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGAc                                          <  2:583387/139‑1 (MQ=255)

                                                                       cGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCgg                                      <  1:718820/139‑1 (MQ=255)

                                                                         cccGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCa                                    <  1:411966/139‑1 (MQ=255)

                                                                              gcCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               >  1:689479/1‑75 (MQ=255)

                                                                              gcCATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCa                                                                                               <  2:689479/75‑1 (MQ=255)

                                                                               ccATTGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAAttt                              <  1:235024/139‑1 (MQ=255)

                                                                                  ttGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     >  2:395769/1‑97 (MQ=255)

                                                                                  ttGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt                                                                     <  1:395769/97‑1 (MQ=255)

                                                                                  ttGCCCATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTaca                           <  1:347610/139‑1 (MQ=255)

                                                                                     cccATCTTGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGt                        <  2:51545/139‑1 (MQ=255)

                                                                                            tGGAGGCAGTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACAtgtg                 <  1:505735/139‑1 (MQ=255)

                                                                                                    gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGcc                                                     >  2:61682/1‑95 (MQ=255)

                                                                                                    gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGcc                                                     <  1:61682/95‑1 (MQ=255)

                                                                                                    gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGaagc            <  1:268277/136‑1 (MQ=255)

                                                                                                    gTGCCACAGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGaagc            >  2:268277/1‑136 (MQ=255)

                                                                                                           aGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGTAGAATTTACACGTACATGTGGAAGCAAGCGGTGAg  <  1:327458/139‑1 (MQ=255)

                                                                                                           aGCGTTCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAg  <  2:580451/139‑1 (MQ=255)

CAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAGAAGCCAAAGACTGGACCCGGCAGAATTTACACGTACATGTGGAAGCAAGCGGTGAG  >  NC_000913/1806430‑1806675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A