Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A10 F1 I1 R1
|
77 |
35.1 |
1569012 |
96.6% |
1515665 |
112.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,051,507 |
G→A |
L271L (CTG→TTG) |
ubiI ← |
2‑octaprenylphenol hydroxylase, FAD‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,051,507 | 0 | G | A | 100.0%
| 77.3
/ NA
| 23 | L271L (CTG→TTG) | ubiI | 2‑octaprenylphenol hydroxylase, FAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/11); total (12/11) |
TTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGA > NC_000913/3051373‑3051641
|
ttCTGCAATCACCTCTGCAACACCCATAAGACCGAGATTTACCCCCGGCCCCGCCAGCGGGTTAATGGTATGTGCGTCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATggg < 2:513034/139‑1 (MQ=255)
tCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCaa > 1:226075/1‑139 (MQ=255)
aGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCa < 2:704875/139‑1 (MQ=255)
aTTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGccc > 1:673977/1‑139 (MQ=255)
cccGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa < 1:16167/139‑1 (MQ=255)
aaTGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa < 1:510877/125‑1 (MQ=255)
aaTGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCaa > 2:510877/1‑125 (MQ=255)
tgtgCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATggg < 1:665084/69‑1 (MQ=255)
tgtgCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATggg > 2:665084/1‑69 (MQ=255)
gcggcgTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTaa > 2:692842/1‑139 (MQ=255)
cgTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTc > 1:78449/1‑139 (MQ=255)
cgTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTc > 1:208422/1‑139 (MQ=255)
tCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGt < 2:171187/139‑1 (MQ=255)
cccACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCtt > 2:531087/1‑139 (MQ=255)
gcgcCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGGTTAAATTCGTCTTCACCTGc < 1:531087/139‑1 (MQ=255)
gACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCctgctg > 2:391323/1‑139 (MQ=255)
tGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATcc > 2:138299/1‑139 (MQ=255)
gCGAGGCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCg < 1:138299/139‑1 (MQ=255)
gCAAACTGGCGCGCATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTgcg > 1:67842/1‑139 (MQ=255)
cgcgcATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCAt < 2:301496/54‑1 (MQ=255)
cgcgcATAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCAt > 1:301496/1‑54 (MQ=255)
tAACGCCCCGTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACa < 1:211836/139‑1 (MQ=255)
gTCAATGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGa < 2:67842/139‑1 (MQ=255)
|
TTCGGCAATCAGCTCTGCAGCATCCATAAAGCCGAGATTTACCCCCTGCCCCGCCAGCGGGTGAATGGTATGTGCGGCGTCGCCCACCAGCGCCAGACGGTGCGAGGCAAACTGGCGCGCATAACGCCCCGTCAGTGGGAACACCTGACGCGCGCTCTCAACCTTGCATAAGCCCAGGCGATTATCAAAAGCGATATTTAACGCGCGATTAAATTCGTCTTCACTTGCCTGCTGCATCCGCTGCGCTTCCTCTGGCGACAGTGACCAGA > NC_000913/3051373‑3051641
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
