Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I1 R1	82	28.6	1225488	97.4%	1193625	116.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,055,285	T→C	T54A (ACG→GCG)	ygfB ←	UPF0149 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,055,285	0	T	C	100.0%	113.2 / NA	30	T54A (ACG→GCG)	ygfB	UPF0149 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/15); total (15/15)

CATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGTCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATACTGGT  >  NC_000913/3055162‑3055404

catcGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAg                                                                                                          <  2:334401/139‑1 (MQ=255)

   cGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCgg                                                                                                       <  1:327395/139‑1 (MQ=255)

      cATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAg                                                                                                    >  1:509199/1‑139 (MQ=255)

            gCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGa                                                                                              <  1:64188/139‑1 (MQ=255)

             cAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAg                                                                                             <  1:605845/139‑1 (MQ=255)

                         aaaaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTa                                                                                 <  1:561272/139‑1 (MQ=255)

                         aaaaaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCC‑TCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTAc                                                                                <  1:158954/139‑1 (MQ=255)

                           aaaGGAAGCCGTCATCCTGCAGGACATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTAcc                                                                               <  2:553234/139‑1 (MQ=255)

                            aaGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCg                                                                              <  2:301553/139‑1 (MQ=255)

                              ggAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGcc                                                                            >  1:299576/1‑139 (MQ=255)

                                aaGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCcac                                                                          >  2:238738/1‑139 (MQ=255)

                                      tCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGACGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATatca                                                                    <  2:399373/139‑1 (MQ=255)

                                              cAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTg                                                            >  2:15051/1‑139 (MQ=255)

                                                 ggCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGAtt                                                         >  1:490716/1‑139 (MQ=255)

                                                       gCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCa                                                   <  1:478271/139‑1 (MQ=255)

                                                           gtggCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCa                                               >  2:121274/1‑139 (MQ=255)

                                                                agagTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCa                                          >  2:531710/1‑139 (MQ=255)

                                                                       aTTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTgggg                                   <  2:299576/139‑1 (MQ=255)

                                                                              gCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCAT‑CCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGAccc                           >  2:547/1‑139 (MQ=255)

                                                                               cAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGAccc                           <  1:531710/139‑1 (MQ=255)

                                                                                 gTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGt                         >  2:577626/1‑139 (MQ=255)

                                                                                  tGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTc                        >  1:159374/1‑139 (MQ=255)

                                                                                       gtgCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCttg                   >  1:114258/1‑139 (MQ=255)

                                                                                        tgCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCttgt                  <  2:490716/139‑1 (MQ=255)

                                                                                        tgCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCttgt                  <  1:15051/139‑1 (MQ=255)

                                                                                              cTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTc            >  2:32635/1‑139 (MQ=255)

                                                                                              cTCATGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTc            >  1:155141/1‑139 (MQ=255)

                                                                                                  tGACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGAt        >  1:78617/1‑139 (MQ=255)

                                                                                                   gACCGAAAGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATa       <  1:238738/139‑1 (MQ=255)

                                                                                                        aaaGCCATGCCTTCGTTCGCCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATACTGGt  >  1:193498/1‑139 (MQ=255)

CATCGCCATCAGGCAGATAAAGCTGAAAAAGGAAGCCGTCATCCTGCAGGGCATCGCTGGTGGCAGAGTGCATTTTACGCAGTGCCTGTGCCAGCTCATGACCGAAAGCCATGCCTTCGTTCGTCAGGTCGTGAAGTAGCGGTAGCCATGAGCTGTCATCGTTACCGCCACATATCATCCCGCTGATTAAACCATGCATCTCAGCTGGGGTCAGACCCGTCCCTTGTTGGTTCAGATACTGGT  >  NC_000913/3055162‑3055404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A