Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F1 I1 R1	77	35.1	1569012	96.6%	1515665	112.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,385,643	T→C	L84S (TTA→TCA)	yjeN →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,385,643	0	T	C	100.0%	123.7 / NA	34	L84S (TTA→TCA)	yjeN	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/17); total (17/17)

TAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATC  >  NC_000913/4385539‑4385769

tAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCtat                                                                                              >  2:681121/1‑139 (MQ=255)

   cACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGc                                                                                           >  1:498650/1‑139 (MQ=255)

   cACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGc                                                                                           >  2:185506/1‑139 (MQ=255)

   cACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGc                                                                                           >  1:346591/1‑139 (MQ=255)

     ccATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGa                                                                                         <  2:498650/139‑1 (MQ=255)

             acCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGc                                                                                 <  2:346591/139‑1 (MQ=255)

                 tCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGAcc                                                                             >  2:463714/1‑139 (MQ=255)

                   aaaGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCgg                                                                           >  1:756360/1‑139 (MQ=255)

                    aaGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGt                                                                          <  1:442241/139‑1 (MQ=255)

                    aaGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATGTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGt                                                                          <  1:539281/139‑1 (MQ=255)

                         cgcgATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCt                                                                     >  1:396747/1‑139 (MQ=255)

                             aTGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGc                                                                                 <  2:384242/123‑1 (MQ=255)

                             aTGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGc                                                                                 >  1:384242/1‑123 (MQ=255)

                             aTGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATg                                                                 <  2:143564/139‑1 (MQ=255)

                              tGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGa                                                                <  1:681121/139‑1 (MQ=255)

                               ggTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAg                                                               <  1:463714/139‑1 (MQ=255)

                                gTAAACGGTGGTGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGt                                                              <  1:185506/139‑1 (MQ=255)

                                gTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGt                                                              <  2:756360/139‑1 (MQ=255)

                                        tGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATg                                                                                                    <  1:369929/93‑1 (MQ=255)

                                        tGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATg                                                                                                    >  2:369929/1‑93 (MQ=255)

                                        tGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCAt                                                      >  1:335200/1‑139 (MQ=255)

                                          gggAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                    <  2:335200/139‑1 (MQ=255)

                                                                    cttatcttatCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCACTATAGCGAAATTAAGCGACCGGt                                                                          <  2:529675/91‑1 (MQ=255)

                                                                    cttatcttagCCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGAATTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGt                                                                          >  1:529675/1‑91 (MQ=255)

                                                                          ttatcCGATGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCg                    <  2:615895/139‑1 (MQ=255)

                                                                                 aTGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                    <  2:187873/100‑1 (MQ=255)

                                                                                 aTGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGt                                                    >  1:187873/1‑100 (MQ=255)

                                                                                 aTGTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCctct             >  2:333135/1‑139 (MQ=255)

                                                                                   gTCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTgg           >  1:185944/1‑139 (MQ=255)

                                                                                    tCTGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGa          <  2:185944/139‑1 (MQ=255)

                                                                                      tGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGGGCGCATGTTTGTc                                               <  2:107845/100‑1 (MQ=255)

                                                                                      tGGTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGGGCGCATGTTTGTc                                               >  1:107845/1‑100 (MQ=255)

                                                                                        gTTTTTCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAAc      >  1:160593/1‑139 (MQ=255)

                                                                                            ttCGCGGGGAATCAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATc  >  2:588240/1‑139 (MQ=255)

TAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGGGATACGGCGATTTGTTTCGTATGCGCTATAGCGAAATTAAGCGACCGGTTACCTTATGAGTGCGCGCATGTTTGTCTTATGCTGCATCTGGTTTATTGTGGCGTTCCTCTGGATAACCATC  >  NC_000913/4385539‑4385769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A