Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F1 I2 R1	59	52.6	2411032	94.1%	2268781	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,866,995	C→T	Q30* (CAA→TAA)	yeaG →	protein kinase, endogenous substrate unidentified, autokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,866,995	0	C	T	100.0%	86.6 / NA	26	Q30* (CAA→TAA)	yeaG	protein kinase, endogenous substrate unidentified, autokinase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (16/10); total (16/10)

ATGAACGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGC  >  NC_000913/1866862‑1867128

atGAACGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGAt                                                                                                                                  >  1:476935/1‑139 (MQ=255)

    aCGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCa                                                                                                                              <  2:476935/139‑1 (MQ=255)

    aCGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCa                                                                                                                              <  2:631293/139‑1 (MQ=255)

        ctctTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGc                                                                                                                          >  2:535171/1‑139 (MQ=255)

        ctctTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGc                                                                                                                          >  2:74745/1‑139 (MQ=255)

                           aaCGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAg                                                                                                       <  2:983347/139‑1 (MQ=255)

                                        cATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATgg                                                                                          >  1:1190499/1‑139 (MQ=255)

                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                                                                                  >  2:869268/1‑128 (MQ=255)

                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGt                                                                                  <  1:869268/128‑1 (MQ=255)

                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         <  2:612068/137‑1 (MQ=255)

                                                           aTCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         >  1:612068/1‑137 (MQ=255)

                                                             cACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATgg                                                                     >  1:157399/1‑139 (MQ=255)

                                                                   cGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa                                                               <  1:1070284/139‑1 (MQ=255)

                                                                                   cTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         >  2:193132/1‑113 (MQ=255)

                                                                                   cTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         <  1:193132/113‑1 (MQ=255)

                                                                                                     tCACACTGCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTc                             >  2:963301/1‑139 (MQ=255)

                                                                                                            gCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGa                                                                                >  1:1118851/1‑81 (MQ=255)

                                                                                                            gCAGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGa                                                                                <  2:1118851/81‑1 (MQ=255)

                                                                                                              aGGAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGt                    >  2:849678/1‑139 (MQ=255)

                                                                                                                taGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATa                                                               >  2:755233/2‑94 (MQ=255)

                                                                                                                gAGTTTCTTACCACTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGTGCCTGTCATGGTCGATa                                                               <  1:755233/94‑1 (MQ=255)

                                                                                                                             ctagacGGTAATATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         >  2:701281/6‑71 (MQ=255)

                                                                                                                             cTTGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTc                                                                         <  1:701281/71‑1 (MQ=255)

                                                                                                                               tGTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGTGACTATTGATGCCTATCGGTGAACCTGTCATGGTCGATCCAGCCCAGGAACCCAGACTTACTCGCCTCCTTTCTAACCGGGTCATTGCCCGTTATCCgg   >  2:744935/1‑139 (MQ=255)

                                                                                                                                gTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGc  >  2:463463/1‑139 (MQ=255)

                                                                                                                                gTCGGTAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGc  >  1:865155/1‑139 (MQ=255)

ATGAACGGCTCTTAACCTGTGCTAAAAAACGAAAGGACGGCATACCATGAATATATTCGATCACTATCGCCAGCGATATGAAGCTGCCAAGGACGAAGAGTTCACACTGCAGGAGTTTCTTACCACTTGTCGGCAAGATCGCAGTGCTTATGCCAACGCGGCTGAGCGGCTATTGATGGCTATCGGTGAGCCTGTCATGGTCGATACAGCCCAGGAACCCAGACTTTCTCGACTCTTTTCTAACCGGGTCATTGCACGTTATCCGGC  >  NC_000913/1866862‑1867128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A