Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F1 I1 R1	71	51.6	2219666	95.9%	2128659	118.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,869,266	G→A	Q104Q (CAG→CAA)	yeaH →	UPF0229 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,869,266	0	G	A	100.0%	117.9 / NA	34	Q104Q (CAG→CAA)	yeaH	UPF0229 family protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/17); total (17/17)

CGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCG  >  NC_000913/1869134‑1869393

cGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCACCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAgccagc                                                                                                                           >  1:1009788/1‑139 (MQ=255)

   aCCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGccagccag                                                                                                                        <  2:320262/139‑1 (MQ=255)

    ccGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAgg                                                                                                                       >  1:497491/1‑139 (MQ=255)

     cGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGa                                                                                                                      <  1:1074601/139‑1 (MQ=255)

                    ggCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGa                                                                                                       <  2:345235/139‑1 (MQ=255)

                                            tGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGaa                                                                               >  2:1103624/1‑139 (MQ=255)

                                                           aCCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGa                                                                >  1:435249/1‑139 (MQ=255)

                                                            ccATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGAt                                                               <  2:894132/139‑1 (MQ=255)

                                                               tttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGa                                                                         <  2:925404/126‑1 (MQ=255)

                                                               tttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGa                                                                         >  1:925404/1‑126 (MQ=255)

                                                               tttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTg                                                            <  2:854729/139‑1 (MQ=255)

                                                                ttCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGc                                                           >  2:1012654/1‑139 (MQ=255)

                                                                             aCCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGAttt                                              >  2:106070/1‑139 (MQ=255)

                                                                                gAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGc                                           <  1:106070/139‑1 (MQ=255)

                                                                                      aaCGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTAcc                                     >  2:1031979/1‑139 (MQ=255)

                                                                                        cGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTg                                                                                                                 <  2:243961/61‑1 (MQ=255)

                                                                                        cGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTg                                                                                                                 >  1:243961/1‑61 (MQ=255)

                                                                                        cGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGa                                   >  2:919263/1‑139 (MQ=255)

                                                                                            cccAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGaa                                                                               <  1:915368/91‑1 (MQ=255)

                                                                                            cccAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGaa                                                                               >  2:915368/1‑91 (MQ=255)

                                                                                                  gTGGCGGCGGAGGTTCCGGGAGTTGGCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACa                         <  2:744795/139‑1 (MQ=255)

                                                                                                     gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAg                                                                                       >  1:797792/1‑74 (MQ=255)

                                                                                                     gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAg                                                                                       <  2:797792/74‑1 (MQ=255)

                                                                                                     gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGc                                           >  1:730598/1‑118 (MQ=255)

                                                                                                     gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGc                                           <  2:730598/118‑1 (MQ=255)

                                                                                                     gcggcgGAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACaaaa                      >  1:390157/1‑139 (MQ=255)

                                                                                                      cggcggAGGTTCCGGCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAAc                     >  2:371029/1‑139 (MQ=255)

                                                                                                                   ggCAGTGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGc        <  1:89441/139‑1 (MQ=255)

                                                                                                                        tGGTCAGGTCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  1:1012654/139‑1 (MQ=255)

                                                                                                                        tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  1:894815/139‑1 (MQ=255)

                                                                                                                        tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   >  2:647233/1‑139 (MQ=255)

                                                                                                                        tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  1:371029/139‑1 (MQ=255)

                                                                                                                        tGGTCAGGGCCAAGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGAcc   <  2:270713/139‑1 (MQ=255)

                                                                                                                         gggcAGGGCCAAGCCAGCCAGGATGGTGAAGGACAGGATGACTTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAAAAACGCCAGCTGACCg  >  1:1046318/4‑139 (MQ=255)

                                                                                                                               ggccaagccaGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATc                                                                    >  1:244454/1‑67 (MQ=255)

                                                                                                                               ggccaagccaGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATc                                                                    <  2:244454/67‑1 (MQ=255)

CGAACCGATGTTTCATCAGGGGCGTGGCGGTCTGCGCCACCGCGTGCATCCGGGCAATGACCATTTCGTCCAGAACGACCGAATTGAACGTCCCCAGGGTGGCGGCGGAGGTTCCGGCAGTGGTCAGGGCCAGGCCAGCCAGGATGGTGAAGGTCAGGATGAATTTGTCTTTCAGATTTCGAAAGATGAGTATCTTGATCTGCTCTTTGAAGATTTGGCCTTACCGAATCTGAAACAAAACCAACAACGCCAGCTGACCG  >  NC_000913/1869134‑1869393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A