Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I2 R1
|
59 |
52.6 |
2411032 |
94.1% |
2268781 |
113.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,981,182 |
C→T |
R144Q (CGG→CAG) |
otsA ← |
trehalose‑6‑phosphate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,981,182 | 0 | C | T | 100.0%
| 126.6
/ NA
| 37 | R144Q (CGG→CAG) | otsA | trehalose‑6‑phosphate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/20); total (17/20) |
TCTGGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTAGATAGCCGTCCCAGGCAG > NC_000913/1981054‑1981311
|
tctgGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTaa > 1:986518/1‑139 (MQ=255)
cAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCa > 2:850257/1‑139 (MQ=255)
cATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa < 2:986518/139‑1 (MQ=255)
cATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa < 1:722747/139‑1 (MQ=255)
aTAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa > 2:1194811/1‑138 (MQ=255)
aTAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa < 1:1194811/138‑1 (MQ=255)
aaaGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATa > 1:172081/1‑139 (MQ=255)
aaGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATaa > 1:911018/1‑139 (MQ=255)
aGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAAt > 1:176414/1‑139 (MQ=255)
ttCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTgg < 1:1112380/139‑1 (MQ=255)
ttCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTgg > 1:1029532/1‑139 (MQ=255)
aagcaagGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATc < 2:758507/139‑1 (MQ=255)
gcaagGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGCAGAAAGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGa < 2:893233/139‑1 (MQ=255)
gtgtCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa > 1:1130981/1‑113 (MQ=255)
gtgtCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCaa < 2:1130981/113‑1 (MQ=255)
tatGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATACGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCAt < 2:172081/139‑1 (MQ=255)
gTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGa < 2:1145355/111‑1 (MQ=255)
gTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGa > 1:1145355/1‑111 (MQ=255)
cGGCAGCGCGTTGAAGATTTACGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGAAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCt > 2:1115619/1‑139 (MQ=255)
gCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATgcg < 1:276929/90‑1 (MQ=255)
gCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATgcg > 2:276929/1‑90 (MQ=255)
cgcgTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAAc > 2:555640/1‑139 (MQ=255)
cgTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAg > 2:529476/1‑139 (MQ=255)
tGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCgg < 1:529476/139‑1 (MQ=255)
tGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCgg < 1:555640/139‑1 (MQ=255)
tGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCgg < 2:1029532/139‑1 (MQ=255)
gAAGATTTACGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGAAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGc < 1:1115619/139‑1 (MQ=255)
tCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAAt > 1:173431/1‑138 (MQ=255)
tCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAAt < 2:173431/138‑1 (MQ=255)
cGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTa < 2:862680/139‑1 (MQ=255)
aagaaaCCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTa < 1:577395/139‑1 (MQ=255)
aaCCAATGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTAGATa > 1:1189277/1‑139 (MQ=255)
tGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCaa > 1:793938/1‑39 (MQ=255)
tGCGATTATTCACTCCCTGTTTGCGTAATTCATGCGCaa < 2:793938/39‑1 (MQ=255)
ttCACTCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCACCAACGCATTTACGCGTAGATAGCCGTCCcaggcag < 1:850257/139‑1 (MQ=255)
tCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCAtt < 2:949817/108‑1 (MQ=255)
tCCCTGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCAtt > 1:949817/1‑108 (MQ=255)
|
TCTGGAAACCCAGCAAATCATAATCACAAAGCTGTTCAAGCAAGGTGTCATATGTCGGCAGCGCGTTGAAGATTTCCGGTGTCGGGAAAGGAATATGC‑‑‑‑AGAAAGAAACCAATGCGATTATTCACTCCCCGTTTGCGTAATTCATGCGCAAATGGCAACAGGTGATAATCGTGGATCCAGATAATGTCATCGTCTTGCAACAGCGGCAGTAATTTATCTGCCAGCAACGCATTTACGCGTAGATAGCCGTCCCAGGCAG > NC_000913/1981054‑1981311
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
