Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F1 I2 R1
|
58 |
36.1 |
1832140 |
90.7% |
1661750 |
105.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,571,850 |
T→C |
T66A (ACC→GCC) |
gadB ← |
glutamate decarboxylase B, PLP‑dependent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,571,850 | 0 | T | C | 100.0%
| 53.7
/ NA
| 15 | T66A (ACC→GCC) | gadB | glutamate decarboxylase B, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/8); total (7/8) |
AGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTG > NC_000913/1571726‑1571966
|
aGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTgg < 1:731982/139‑1 (MQ=255)
aTCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc < 1:411543/139‑1 (MQ=255)
aTCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc < 2:349218/139‑1 (MQ=255)
cgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCAt < 1:802436/139‑1 (MQ=255)
ttGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGa < 1:806707/93‑1 (MQ=255)
ttGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGa > 2:806707/1‑93 (MQ=255)
tGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc > 1:89758/1‑103 (MQ=255)
tGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc < 2:89758/103‑1 (MQ=255)
tGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGACCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGAc > 2:562518/1‑139 (MQ=255)
tGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGc > 1:451885/1‑48 (MQ=255)
tGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGc < 2:451885/48‑1 (MQ=255)
tGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAg > 2:797285/1‑139 (MQ=255)
tCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc > 1:400157/1‑47 (MQ=255)
tCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc < 2:400157/47‑1 (MQ=255)
tgGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTg > 2:549440/1‑139 (MQ=255)
|
AGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTG > NC_000913/1571726‑1571966
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
