Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F1 I2 R1	97	53.3	2484976	94.5%	2348302	111.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,857,841	T→C	S331S (TCA→TCG)	ydjJ ←	putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,857,841	0	T	C	98.6%	265.7 / NA	71	S331S (TCA→TCG)	ydjJ	putative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (38/32); minor base A (0/1); total (38/33)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGGGCAATTGCATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACTGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGG  >  NC_000913/1857706‑1857961

aaTGGGCAATTGCATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTATTAATGTCGCGTTTGTTGTTAACCGAc                                                                                                                       >  1:1083351/1‑139 (MQ=255)

      cAATTGCATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCa                                                                                                                 <  1:602357/139‑1 (MQ=255)

         ttGCATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAAt                                                                                                              <  1:323863/139‑1 (MQ=255)

           gCATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGc                                                                                                            <  1:108936/139‑1 (MQ=255)

            cATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGcc                                                                                                           <  2:286537/139‑1 (MQ=255)

            cATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGcc                                                                                                           <  2:200931/139‑1 (MQ=255)

                   tttATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTa                                                                                                    >  2:796178/1‑139 (MQ=255)

                      aTTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACAt                                                                                                 >  1:631895/1‑139 (MQ=255)

                      aTTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACAt                                                                                                 >  1:1185706/1‑139 (MQ=255)

                      aTTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACAt                                                                                                 >  2:929450/1‑139 (MQ=255)

                           tgGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGa                                                                                            >  2:394494/1‑139 (MQ=255)

                           tgGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGa                                                                                            >  2:424426/1‑139 (MQ=255)

                           tgGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGa                                                                                            >  2:883537/1‑139 (MQ=255)

                            ggCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTa                                                                                                    <  2:548603/130‑1 (MQ=255)

                            ggCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTa                                                                                                    >  1:548603/1‑130 (MQ=255)

                            ggCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGAt                                                                                           <  2:96583/139‑1 (MQ=255)

                            ggCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGAt                                                                                           <  1:1168502/139‑1 (MQ=255)

                            ggCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGAt                                                                                           <  1:796178/139‑1 (MQ=255)

                            ggCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGAt                                                                                           >  1:469651/1‑139 (MQ=255)

                                  gTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGt                                                                                     >  1:492631/1‑139 (MQ=255)

                                       cccAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAata                                                                                <  1:394494/139‑1 (MQ=255)

                                         cAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATg                                                                              <  2:47137/139‑1 (MQ=255)

                                            tatctgatatctgCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGt                                                                           >  2:471303/1‑139 (MQ=255)

                                                tgatatctgCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCAcc                                                                       <  1:449426/139‑1 (MQ=255)

                                                tgatatctgCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCAcc                                                                       <  2:573115/139‑1 (MQ=255)

                                                  atatctgCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCAt                                                                     >  1:1131722/1‑139 (MQ=255)

                                                       tgCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGAtt                                                                >  1:379198/1‑139 (MQ=255)

                                                        gCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCt                                                                                                          <  1:978702/96‑1 (MQ=255)

                                                        gCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCt                                                                                                          >  2:978702/1‑96 (MQ=255)

                                                        gCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGAttt                                                               <  1:424426/139‑1 (MQ=255)

                                                         cGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATc                                                                                       <  1:741284/114‑1 (MQ=255)

                                                         cGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTGGTACATCCCGATAATc                                                                                       >  2:741284/1‑114 (MQ=255)

                                                           aGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAAt                                                                                                              <  1:395/89‑1 (MQ=255)

                                                           aGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAAt                                                                                                              >  2:395/1‑89 (MQ=255)

                                                            gCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTcaca                                                           >  2:983424/1‑139 (MQ=255)

                                                                 ttctgttctCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGaa                                                      <  2:631895/139‑1 (MQ=255)

                                                                 ttctgttctCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGaa                                                      >  2:1180373/1‑139 (MQ=255)

                                                                      ttctCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGcc                                                 <  1:883537/139‑1 (MQ=255)

                                                                      ttctCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGcc                                                 <  1:929450/139‑1 (MQ=255)

                                                                      ttctCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGcc                                                 >  1:216712/1‑139 (MQ=255)

                                                                         taccGTATTAATTAATCGCTAATTTTAATATCGGCTTTACTACTGTCGCGTTTGTTGTTTATAAGCTCTTCAAATTACTGTTGTACATCCCGATTATCGTAAATAGGCGTCACCATCGATTTCACATCGAATCGCCCTg                                              <  2:1185706/137‑1 (MQ=255)

                                                                           ccGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTgaa                                            >  2:434407/1‑139 (MQ=255)

                                                                              tATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGa                                                                                            <  1:426063/88‑1 (MQ=255)

                                                                              tATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGa                                                                                            >  2:426063/1‑88 (MQ=255)

                                                                                        tCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGAtt                                                                >  1:428802/1‑106 (MQ=255)

                                                                                        tCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGAtt                                                                <  2:428802/106‑1 (MQ=255)

                                                                                        tCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGaa                                                      <  1:967112/116‑1 (MQ=255)

                                                                                        tCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGaa                                                      >  2:967112/1‑116 (MQ=255)

                                                                                        tCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGccc                                                <  2:823678/122‑1 (MQ=255)

                                                                                        tCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGccc                                                >  1:823678/1‑122 (MQ=255)

                                                                                              aTTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGa                         >  1:311588/1‑139 (MQ=255)

                                                                                                     taaCGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATa                  <  1:733666/139‑1 (MQ=255)

                                                                                                        cGCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACg               >  2:1006406/1‑139 (MQ=255)

                                                                                                         gCCTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGa              >  1:89849/1‑139 (MQ=255)

                                                                                                          ccTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGAt             >  2:1108309/1‑139 (MQ=255)

                                                                                                          ccTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGAt             >  1:383913/1‑139 (MQ=255)

                                                                                                          ccTTTAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGAt             >  1:507274/1‑139 (MQ=255)

                                                                                                             ttAATAATGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTgg          <  2:507274/139‑1 (MQ=255)

                                                                                                                 taatGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATa      <  2:1083351/139‑1 (MQ=255)

                                                                                                                  aatGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAg     <  1:1108309/139‑1 (MQ=255)

                                                                                                                   atGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGc    <  2:383913/139‑1 (MQ=255)

                                                                                                                    tGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTGAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCg   >  2:871154/1‑139 (MQ=255)

                                                                                                                    tGTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCg   >  2:631506/1‑139 (MQ=255)

                                                                                                                     gTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCt                                                                                                          <  1:1017728/35‑1 (MQ=255)

                                                                                                                     gTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCt                                                                                                          >  2:1017728/1‑35 (MQ=255)

                                                                                                                     gTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATc                                                                                                >  2:1196984/1‑45 (MQ=255)

                                                                                                                     gTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATc                                                                                                <  1:1196984/45‑1 (MQ=255)

                                                                                                                     gTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCgg  >  1:102101/1‑139 (MQ=255)

                                                                                                                     gTCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCgg  >  1:212626/1‑139 (MQ=255)

                                                                                                                      tCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCAt                                                                     <  1:336898/71‑1 (MQ=255)

                                                                                                                      tCGCGTTTGTTGTTAACCGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCAt                                                                     >  2:336898/1‑71 (MQ=255)

AATGGGCAATTGCATAATGTTTATTTGTGGCATCGTTTTCCCAATATCTGATATCTGCGAGCATGTTCTGTTCTCCCGTATTAATTAATCGCTAATTTTAATAACGCCTTTAATAATGTCGCGTTTGTTGTTAACTGACTCTTCAAATGCCTGTTGTACATCCCGATAATCGTAAATATGCGTCACCATCGATTTCACATCGAATCGCCCTGAAGAAATAGCTTCAATCGTGACCGGATAACGATTGGCATAGCGG  >  NC_000913/1857706‑1857961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A