Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F1 I2 R1
|
97 |
53.3 |
2484976 |
94.5% |
2348302 |
111.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,335,018 |
G→A |
L543L (CTG→TTG) |
yfaA ← |
DUF2138 family protein, putative host defense protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,335,018 | 0 | G | A | 100.0%
| 89.7
/ NA
| 27 | L543L (CTG→TTG) | yfaA | DUF2138 family protein, putative host defense protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/13); total (14/13) |
AGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAG > NC_000913/2334895‑2335144
|
aGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAAcg > 1:425114/1‑139 (MQ=255)
aGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAAcg > 2:231870/1‑139 (MQ=255)
cGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGgttgt > 2:1113293/1‑139 (MQ=255)
ggCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATa > 2:789223/1‑139 (MQ=255)
ggCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATa < 1:464333/139‑1 (MQ=255)
caacacaacaCAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCg < 2:871391/139‑1 (MQ=255)
acacaacacaGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTc < 1:789223/139‑1 (MQ=255)
agccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCgg < 2:870584/139‑1 (MQ=255)
agccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCgg > 1:493369/1‑139 (MQ=255)
ccagcAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCa > 1:317725/1‑139 (MQ=255)
cccGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGt < 2:425114/139‑1 (MQ=255)
tGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTgcgg < 1:1235104/132‑1 (MQ=255)
tGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTgcgg > 2:1235104/1‑132 (MQ=255)
gCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACa > 2:639038/1‑62 (MQ=255)
gCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACa < 1:639038/62‑1 (MQ=255)
ccAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTACCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCAGTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCgaga > 1:827905/1‑139 (MQ=255)
gggTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGc < 1:1113293/139‑1 (MQ=255)
gTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAg < 2:493369/139‑1 (MQ=255)
tAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGa < 2:317725/139‑1 (MQ=255)
gCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACt > 1:1068826/1‑139 (MQ=255)
gCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACt > 1:427504/1‑139 (MQ=255)
cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTcc > 2:227806/1‑59 (MQ=255)
cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTcc < 1:227806/59‑1 (MQ=255)
cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGt > 2:368349/1‑90 (MQ=255)
cACCGGGTTCCATCTGGGCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGt < 1:368349/90‑1 (MQ=255)
ggCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGcagcag < 1:489410/132‑1 (MQ=255)
ggCCAACTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGcagcag > 2:489410/1‑132 (MQ=255)
|
AGCATTTCGCTATGGGCAACAACACAACACAGCCAGCAAATCAGCGCCAGCAGCCCGTGCCTCATAGCGGCTGCCAGGTTATGGGTAGCCACTGCCAGGCGGCACCGGGTTCCATCTGGGCCAGCTTCATGACATAACGCGGTTGTTGAGATAAAGCGTCCAGCTTCGGCATTAATAAAGTTTGTGCGGCGTTATAAAAAACCGGTTCGAGATTCTTCGGCAGACTGGTCAACGTTTCGTTACGCAGCAG > NC_000913/2334895‑2335144
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
