Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F1 I2 R1 97 53.3 2484976 94.5% 2348302 111.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,691,927 G→A A1206V (GCG→GTG)  purL ← phosphoribosylformyl‑glycineamide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,691,9270GA100.0% 203.8 / NA 58A1206V (GCG→GTG) purLphosphoribosylformyl‑glycineamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

CAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGG  >  NC_000913/2691796‑2692059
                                                                                                                                   |                                                                                                                                    
cAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACg                                                                                                                               <  2:208418/139‑1 (MQ=255)
   tCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCAc                                                                                                                            >  1:306322/1‑139 (MQ=255)
      tGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              >  2:481466/1‑134 (MQ=255)
      tGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              <  1:481466/134‑1 (MQ=255)
          tGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcg                                                                                                                     <  2:1127671/139‑1 (MQ=255)
          tGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcg                                                                                                                     <  1:872683/139‑1 (MQ=255)
               tAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGccc                                                                                                                <  1:917943/139‑1 (MQ=255)
                      gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              <  1:757596/118‑1 (MQ=255)
                      gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGc                                                                                                                              >  2:757596/1‑118 (MQ=255)
                      gTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCa                                                                                                         >  1:780957/1‑139 (MQ=255)
                       ttCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCAt                                                                                                        >  2:620897/1‑139 (MQ=255)
                        tCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcgcg                                                                                                                   >  2:181835/1‑127 (MQ=255)
                        tCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCAcgcg                                                                                                                   <  1:181835/127‑1 (MQ=255)
                        tCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATg                                                                                                       <  2:363475/139‑1 (MQ=255)
                                  gTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCa                                                                                             <  1:620897/139‑1 (MQ=255)
                                   ttCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCaa                                                                                            >  2:1162580/1‑139 (MQ=255)
                                             ccGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTg                                                                                  >  1:575906/1‑139 (MQ=255)
                                                 gTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAACAATCGGCATTTGCGAg                                                                              <  2:795551/139‑1 (MQ=255)
                                                       ttCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCccacc                                                                        >  1:596538/1‑139 (MQ=255)
                                                       ttCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCccacc                                                                        <  2:780957/139‑1 (MQ=255)
                                                           gTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATcc                                                                    >  1:1097454/1‑139 (MQ=255)
                                                                tttGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGc                                                                             >  2:327444/1‑125 (MQ=255)
                                                                tttGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGc                                                                             <  1:327444/125‑1 (MQ=255)
                                                                tttGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGc                                                               <  2:1097454/139‑1 (MQ=255)
                                                                            aTCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  1:837379/1‑111 (MQ=255)
                                                                            aTCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  2:837379/111‑1 (MQ=255)
                                                                             tCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACg                                                  >  1:543765/1‑139 (MQ=255)
                                                                                    aGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  2:1232694/1‑103 (MQ=255)
                                                                                    aGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  1:1232694/103‑1 (MQ=255)
                                                                                    aGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTg                                           >  1:649141/1‑139 (MQ=255)
                                                                                     gcgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTgg                                          <  2:543765/139‑1 (MQ=255)
                                                                                     gcgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTgg                                          <  2:142906/139‑1 (MQ=255)
                                                                                      cgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCtt                                                                                                              >  2:479860/1‑70 (MQ=255)
                                                                                      cgcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCtt                                                                                                              <  1:479860/70‑1 (MQ=255)
                                                                                       gcAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGt                                        <  2:936798/139‑1 (MQ=255)
                                                                                           tGCCACCAGCCCTTTGCTTTCCAGTGCGGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACt                                    >  1:801042/1‑139 (MQ=255)
                                                                                           tGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACt                                    >  2:947404/1‑139 (MQ=255)
                                                                                           tGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACt                                    >  1:93872/1‑139 (MQ=255)
                                                                                            gCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATcc                                                                    <  1:544500/106‑1 (MQ=255)
                                                                                            gCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATcc                                                                    >  2:544500/1‑106 (MQ=255)
                                                                                             ccaccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  2:1132222/94‑1 (MQ=255)
                                                                                             ccaccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  1:1132222/1‑94 (MQ=255)
                                                                                               accaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCaa                                <  1:831353/139‑1 (MQ=255)
                                                                                                ccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAAc                               <  2:649141/139‑1 (MQ=255)
                                                                                                ccaGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAAc                               >  1:361114/1‑139 (MQ=255)
                                                                                                     ccTTTGCTTTCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGc                          >  1:894496/1‑139 (MQ=255)
                                                                                                            tttCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAAcg                   <  2:393408/139‑1 (MQ=255)
                                                                                                              tCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               <  1:623723/77‑1 (MQ=255)
                                                                                                              tCCAGTGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGa                                                                               >  2:623723/1‑77 (MQ=255)
                                                                                                                   tGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAg                                                                                               <  2:941359/56‑1 (MQ=255)
                                                                                                                   tGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAg                                                                                               >  1:941359/1‑56 (MQ=255)
                                                                                                                   tGCCGCCAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCa            <  1:1162580/139‑1 (MQ=255)
                                                                                                                     ccgccAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCaaa          <  2:681760/139‑1 (MQ=255)
                                                                                                                      cgccAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAg         <  1:82930/139‑1 (MQ=255)
                                                                                                                        ccAGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCg       <  2:596538/139‑1 (MQ=255)
                                                                                                                          aGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAt     <  1:947404/139‑1 (MQ=255)
                                                                                                                          aGATGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGAt     <  2:361114/139‑1 (MQ=255)
                                                                                                                             tGCGCCACATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCgg  >  1:1113080/1‑139 (MQ=255)
                                                                                                                                   |                                                                                                                                    
CAGTCGTGACTGCCGTAATACCGTTCGGTGAACCGTTCGGGTTAGCCGGGTAGGTTTCAGTGACTTTGCCGAAGTTATCGACATAGCGCAGTGCCACCAGCCCTTTGCTTTCCAGTGCCGCCAGATGCGCCGCATCACGCACTTCCACGCGCCCTTCACCATGAGAGACAGCAATCGGCATTTGCGAGCCCACCATCCCCTGCAACAGCAGAGACGGGCTTTGGGTTACTTCAACCAGGCTGAAACGCGCTTCAAAGCGATCGG  >  NC_000913/2691796‑2692059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A