Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	77	34.8	1608548	95.0%	1528120	114.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,873,602	C→T	T10M (ACG→ATG)	yeaK →	aminoacyl‑tRNA editing domain protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,873,602	0	C	T	96.7%	197.8 / ‑1.8	61	T10M (ACG→ATG)	yeaK	aminoacyl‑tRNA editing domain protein
Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (31/28); total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCACTG  >  NC_000913/1873468‑1873735

tgCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCAt                                                                                                                                   <  2:509606/139‑1 (MQ=255)

tgCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCAt                                                                                                                                   >  2:165941/1‑139 (MQ=255)

tgCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCAt                                                                                                                                   >  1:307304/1‑139 (MQ=255)

tgCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCAt                                                                                                                                   >  2:676898/1‑139 (MQ=255)

 gCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATc                                                                                                                                  >  2:94055/1‑139 (MQ=255)

  cGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCa                                                                                                                                 >  1:128587/1‑139 (MQ=255)

           cTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAAt                                                                                                                        <  2:458997/139‑1 (MQ=255)

              ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGc                                                                                                                     >  2:73150/1‑139 (MQ=255)

              ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGc                                                                                                                     >  1:306939/1‑139 (MQ=255)

              ccGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGc                                                                                                                     >  1:553551/1‑139 (MQ=255)

                     gCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTttatta                                                                                                              <  2:242234/139‑1 (MQ=255)

                             atGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACaaga                                                                                                      <  1:73150/139‑1 (MQ=255)

                              tGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACaagaa                                                                                                     >  2:774961/1‑139 (MQ=255)

                                tCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAgg                                                                                                   >  2:683898/1‑139 (MQ=255)

                                  aaTAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTg                                                                                                 >  1:131992/1‑139 (MQ=255)

                                   aTAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGc                                                                                                >  2:143008/1‑139 (MQ=255)

                                      aGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGa                                                                                             >  2:277289/1‑139 (MQ=255)

                                      aGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGa                                                                                             >  2:21276/1‑139 (MQ=255)

                                           aaCAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTcc                                                                                        >  2:692987/1‑139 (MQ=255)

                                                     gCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTttat                                                                                                                <  1:421603/105‑1 (MQ=255)

                                                     gCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTttat                                                                                                                >  2:421603/1‑105 (MQ=255)

                                                         ttCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCAt                                                                          <  2:553551/139‑1 (MQ=255)

                                                               aTAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTAGCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCg                                                                    <  1:285597/139‑1 (MQ=255)

                                                               aTAACATTCTGTTGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGTCTGAGATGGCTATAGGAAGCGTGCTGCATCAGCGGATAATCGCTTTATTATCACAAGAAGGTGCAGACTTCCGCGTCGATACCCATGAACCg                                                                    <  1:464219/139‑1 (MQ=255)

                                                                   ccTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCggtag                                                                >  2:470323/1‑139 (MQ=255)

                                                                      tCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCggtaggta                                                             >  2:656109/1‑139 (MQ=255)

                                                                       cTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATc                                                                                                                       <  2:254005/80‑1 (MQ=255)

                                                                       cTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATc                                                                                                                       >  1:254005/1‑80 (MQ=255)

                                                                         gtgGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCAt                                                                                                                                   >  1:132302/1‑66 (MQ=255)

                                                                         gtgGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCAt                                                                                                                                   <  2:132302/66‑1 (MQ=255)

                                                                                  tGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCgg                                                 <  2:128587/139‑1 (MQ=255)

                                                                                      tGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACaagaag                                                                                                    >  1:416980/1‑84 (MQ=255)

                                                                                      tGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACaagaag                                                                                                    <  2:416980/84‑1 (MQ=255)

                                                                                      tGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTc                                             >  2:739041/1‑139 (MQ=255)

                                                                                          aTCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGaa                                         >  2:110854/1‑139 (MQ=255)

                                                                                          aTCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGaa                                         >  2:386589/1‑139 (MQ=255)

                                                                                               aGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCg                                    >  1:437867/1‑139 (MQ=255)

                                                                                               aGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCg                                    >  2:320495/1‑139 (MQ=255)

                                                                                                     gTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAc                              <  1:110854/139‑1 (MQ=255)

                                                                                                     gTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAc                              <  1:739041/139‑1 (MQ=255)

                                                                                                     gTGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCAc                              <  1:21276/139‑1 (MQ=255)

                                                                                                      tGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATg                                                                         >  1:75393/1‑95 (MQ=255)

                                                                                                      tGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATg                                                                         <  2:75393/95‑1 (MQ=255)

                                                                                                      tGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTAAATCGCTTTATTATCACAAGAAGGTGCTGACTTCcgc                                                                                      >  1:295285/1‑82 (MQ=255)

                                                                                                      tGAAATGACTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTAAATCGCTTTATTATCACAAGAAGGTGCTGACTTCcgc                                                                                      <  2:295285/82‑1 (MQ=255)

                                                                                                              cTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCg                                                                    >  1:50232/1‑92 (MQ=255)

                                                                                                              cTGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCg                                                                    <  2:50232/92‑1 (MQ=255)

                                                                                                               tGAAATGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGc                    <  2:477962/139‑1 (MQ=255)

                                                                                                                 aaaTGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCa                  <  1:656109/139‑1 (MQ=255)

                                                                                                                  aaTGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAg                 <  2:306939/139‑1 (MQ=255)

                                                                                                                  aaTGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAg                 <  1:143008/139‑1 (MQ=255)

                                                                                                                   aTGGCTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAgg                <  1:320495/139‑1 (MQ=255)

                                                                                                                       cTAAAGGAAGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGc            <  2:232136/139‑1 (MQ=255)

                                                                                                                              aaGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCa     <  2:131992/139‑1 (MQ=255)

                                                                                                                              aaGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCa     <  1:692987/139‑1 (MQ=255)

                                                                                                                              aaGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCa     <  1:646010/139‑1 (MQ=255)

                                                                                                                               aGCGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCAc    >  2:330386/1‑139 (MQ=255)

                                                                                                                                gCGTGATGCATCAGCGGTTAATCGCTTTATTATCACaagaag                                                                                                    >  2:274510/1‑42 (MQ=255)

                                                                                                                                gCGTGATGCATCAGCGGTTAATCGCTTTATTATCACaagaag                                                                                                    <  1:274510/42‑1 (MQ=255)

                                                                                                                                 cGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCACTg  <  2:667549/139‑1 (MQ=255)

                                                                                                                                 cGTGATGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCACTg  >  1:667549/1‑139 (MQ=255)

TGCGTATAAAGCTTCCGATGAGCGTTTATATGTCAATAAGCAGAACAAAAACAGCCGTTCATGATAACCTTCTGTGGTTGTTTGCTTGTAATCTCAGGAGCGTGAAATGACTGAAATGGCTAAAGGAAGCGTGACGCATCAGCGGTTAATCGCTTTATTATCACAAGAAGGTGCTGACTTCCGCGTCGTTACCCATGAAGCGGTAGGTAAATGTGAAGCGGTGTCAGAAATTCGTGGCACCGCTCTAGGCCAGGGTGCTAAGGCACTG  >  NC_000913/1873468‑1873735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A