Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
77 |
34.8 |
1608548 |
95.0% |
1528120 |
114.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,995,320 |
G→A |
R14C (CGC→TGC) |
uvrY ← |
response regulator in two‑component regulatory system with BarA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,995,320 | 0 | G | A | 100.0%
| 134.9
/ NA
| 39 | R14C (CGC→TGC) | uvrY | response regulator in two‑component regulatory system with BarA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/23); total (16/23) |
ATGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCGCACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAAATGGT > NC_000913/1995196‑1995452
|
aTGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGt < 2:453394/139‑1 (MQ=255)
cATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGtcatca > 1:328762/1‑139 (MQ=255)
taacaccacGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGCTCATcaaca > 2:513734/4‑139 (MQ=255)
tAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac < 1:204198/138‑1 (MQ=255)
tAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac > 2:204198/1‑138 (MQ=255)
accacGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTa < 1:96911/139‑1 (MQ=255)
acGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGaa > 1:453310/1‑139 (MQ=255)
cGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac > 2:444303/1‑130 (MQ=255)
cGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATcaac < 1:444303/130‑1 (MQ=255)
gCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCa < 2:570178/139‑1 (MQ=255)
tGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGa < 2:328762/139‑1 (MQ=255)
caccacTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATAtctc < 1:439120/139‑1 (MQ=255)
ccacTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCa < 1:350892/139‑1 (MQ=255)
ttAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGaaa < 2:92245/139‑1 (MQ=255)
gTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATa < 1:438544/139‑1 (MQ=255)
gCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTg < 1:15182/139‑1 (MQ=255)
tGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtt > 2:693883/1‑139 (MQ=255)
tGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtt > 1:64929/1‑139 (MQ=255)
tGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtt > 1:279567/1‑139 (MQ=255)
cTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagttagt < 1:264322/139‑1 (MQ=255)
cTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagttagt < 1:229404/139‑1 (MQ=255)
aCCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt < 1:513734/139‑1 (MQ=255)
aCCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt < 1:794133/139‑1 (MQ=255)
ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGa < 1:270269/70‑1 (MQ=255)
ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGa > 2:270269/1‑70 (MQ=255)
ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt < 1:479366/99‑1 (MQ=255)
ttCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACt > 2:479366/1‑99 (MQ=255)
tCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt < 1:318002/111‑1 (MQ=255)
tCCAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCAt > 2:318002/1‑111 (MQ=255)
ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGt < 2:92730/39‑1 (MQ=255)
ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGt > 1:92730/1‑39 (MQ=255)
ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtta > 2:573207/1‑105 (MQ=255)
ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagt < 1:88955/103‑1 (MQ=255)
ccAGAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagt > 2:88955/1‑103 (MQ=255)
ccAGAATGCGTCGTATCCCTGCGCACACAAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGAtagtta < 1:573207/105‑1 (MQ=255)
gAATGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGa < 2:453310/139‑1 (MQ=255)
aaTGCGTCGTATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGaa > 1:30627/1‑139 (MQ=255)
cgtcgtATCCCTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGt > 1:11212/1‑139 (MQ=255)
cTGCGCACACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAAAtggt < 1:693883/139‑1 (MQ=255)
|
ATGTCCATTAGCACCACGTCAACGGCATTTGTCCGGCACCACTTAACGGCGTCTTCACCGCACGATGCCTCACCGACGACTTTTATACCCTTTATATCTTCCAGAATGCGTCGTATCCCTGCGCGCACCAGTTCGTGGTCATCAACAAGTAGAACGTTGATCAAAGGAATATCTCCAGAAATAGGGATAACGCTACTGATAGTTAGTCATTCGTATATTAGCGGTTTTTATTGCAACTTTGAAACGTTAAAAATGGT > NC_000913/1995196‑1995452
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
