Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
77 |
34.8 |
1608548 |
95.0% |
1528120 |
114.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,631,531 |
G→A |
G335G (GGC→GGT) |
guaA ← |
GMP synthetase (glutamine aminotransferase) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,631,531 | 0 | G | A | 100.0%
| 40.0
/ NA
| 13 | G335G (GGC→GGT) | guaA | GMP synthetase (glutamine aminotransferase) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (7/6); total (7/6) |
AACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGA > NC_000913/2631397‑2631660
|
aaCAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCt > 2:450112/1‑139 (MQ=255)
aGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTgcg < 1:389870/139‑1 (MQ=255)
ctctTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCgcc > 1:359599/1‑139 (MQ=255)
tttCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCgccagc < 2:359599/139‑1 (MQ=255)
gCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTAcc > 1:250556/1‑124 (MQ=255)
tCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGt > 1:442828/1‑139 (MQ=255)
tCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGt < 2:601264/139‑1 (MQ=255)
aTCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGctt < 1:574669/139‑1 (MQ=255)
atgaCGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAAcg < 1:339930/126‑1 (MQ=255)
atgaCGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAAcg > 2:339930/1‑126 (MQ=255)
tgCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGtttt > 2:249888/1‑139 (MQ=255)
aCGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTc > 1:358160/1‑139 (MQ=255)
gTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGa < 2:442828/139‑1 (MQ=255)
|
AACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAGCGA > NC_000913/2631397‑2631660
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
