Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F1 I1 R1
|
81 |
28.9 |
1316934 |
95.8% |
1261622 |
113.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,634,449 |
G→A |
R73H (CGC→CAC) |
xseA → |
exonuclease VII, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,634,449 | 0 | G | A | 100.0%
| 97.2
/ NA
| 30 | R73H (CGC→CAC) | xseA | exonuclease VII, large subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/15); total (15/15) |
GGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACAGAAGTA > NC_000913/2634318‑2634572
|
ggTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAg > 1:628156/1‑139 (MQ=255)
gcggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGcaaca > 2:311653/1‑139 (MQ=255)
gcggcgAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGcaaca > 1:447503/1‑139 (MQ=255)
ttCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGtt < 2:336915/139‑1 (MQ=255)
cAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTa < 1:536867/139‑1 (MQ=255)
cacTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCcaca < 1:128339/76‑1 (MQ=255)
cacTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCcaca > 2:128339/1‑76 (MQ=255)
cAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACCCTCTACGAGCCGCGCGGCGACTACCAGATa < 1:351573/139‑1 (MQ=255)
aaaGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATaa > 2:310731/1‑139 (MQ=255)
acgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt < 2:628156/139‑1 (MQ=255)
acgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt < 2:447503/139‑1 (MQ=255)
acgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt < 1:601863/139‑1 (MQ=255)
cgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt < 1:418490/138‑1 (MQ=255)
cgacACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt > 2:418490/1‑138 (MQ=255)
cacCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTgag < 2:563041/139‑1 (MQ=255)
cacCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTgag > 1:238040/1‑139 (MQ=255)
gTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAgc > 2:315881/1‑139 (MQ=255)
cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATggg < 1:404011/55‑1 (MQ=255)
cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATggg > 2:404011/1‑55 (MQ=255)
cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt > 1:561595/1‑120 (MQ=255)
cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGt < 2:561595/120‑1 (MQ=255)
cTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAgccggcc > 1:434330/1‑139 (MQ=255)
tGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCgcgc > 1:189961/1‑74 (MQ=255)
tGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCgcgc < 2:189961/74‑1 (MQ=255)
cgcgATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCgcggc < 1:402542/100‑1 (MQ=255)
cgcgATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCgcggc > 2:402542/1‑100 (MQ=255)
cgATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGa > 2:282663/1‑139 (MQ=255)
gCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACa < 1:282663/139‑1 (MQ=255)
gCAACCGCCGGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACa < 2:434330/139‑1 (MQ=255)
gccgGGTGACCTTCCACCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACAGAAGTa > 1:557551/1‑139 (MQ=255)
|
GGTTTGGATCAGCGGCGAAATTTCTAATTTCACGCAACCAGCTTCCGGTCACTGGTACTTTACACTCAAAGACGACACCGCCCAGGTACGCTGCGCGATGTTCCGCAACAGCAACCGCCGGGTGACCTTCCGCCCACAGCATGGGCAACAAGTTTTAGTTCGCGCCAATATTACGCTCTACGAGCCGCGCGGCGACTACCAGATAATCGTTGAGAGTATGCAGCCGGCCGGTGAAGGGCTGCTGCAACAGAAGTA > NC_000913/2634318‑2634572
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
