Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
81 |
47.9 |
2225048 |
94.8% |
2109345 |
113.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,631,531 |
G→A |
G335G (GGC→GGT) |
guaA ← |
GMP synthetase (glutamine aminotransferase) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,631,531 | 0 | G | A | 96.4%
| 87.8
/ ‑4.1
| 28 | G335G (GGC→GGT) | guaA | GMP synthetase (glutamine aminotransferase) |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/16); total (12/16) |
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAG > NC_000913/2631395‑2631657
|
tGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTAcc < 1:358792/139‑1 (MQ=255)
ctctTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCgcc > 1:297172/1‑139 (MQ=255)
tCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCgccagcca > 1:21939/1‑139 (MQ=255)
cGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTc < 2:21939/139‑1 (MQ=255)
ggTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCa > 1:80704/1‑139 (MQ=255)
gTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCAc < 2:970571/139‑1 (MQ=255)
ttCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACg < 2:744379/139‑1 (MQ=255)
tCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGt < 1:664131/139‑1 (MQ=255)
tCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAg < 2:297172/139‑1 (MQ=255)
aTCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGctt < 1:154635/139‑1 (MQ=255)
ctctTTCGTCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttct < 1:851910/139‑1 (MQ=255)
ttCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTc > 1:349577/1‑126 (MQ=255)
ttCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTc < 2:349577/126‑1 (MQ=255)
tCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATc > 2:315919/1‑139 (MQ=255)
cGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCg < 2:12138/139‑1 (MQ=255)
ccgccCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACtt < 2:80704/139‑1 (MQ=255)
gtgAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGa > 2:817237/1‑139 (MQ=255)
tgatgaCGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCgatgat > 2:1030471/1‑139 (MQ=255)
gtgtGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGt > 1:1064659/1‑139 (MQ=255)
cTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGc > 1:787768/1‑139 (MQ=255)
aCCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTcc > 2:744889/1‑139 (MQ=255)
tGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttct < 2:784444/82‑1 (MQ=255)
tGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTACCCTACGCCAGCCACTTCACGTCTTCCAGTTTCAGCGcttct > 1:784444/1‑82 (MQ=255)
gcAGATTCGATAACGTCAGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCgccag < 1:95260/139‑1 (MQ=255)
cgATTCGATAACGTCAGGGTAGATTGTACCCTGCGCCAGCCACCTCACGTCTTCCAGTTTCAGTGCTTCTTCATCGAATACTTCAACGAGAACGCGACCGATTTTTTTACGTTTTGCTTCCGGATCGTTTTCgccagcc < 1:1058600/138‑1 (MQ=255)
aGGGTAGATGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAg < 1:817237/139‑1 (MQ=255)
aTGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCg > 2:550101/1‑78 (MQ=255)
aTGGTACCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCg < 1:550101/78‑1 (MQ=255)
|
TGAACAGCTCTTTCAGCGGTTCAACCAGGCCCATCTTCATCTCTTTCGGCAGGCCGCCCACGTTGTGGTGAGATTTGATGACGTGTGCTTTACCGGTTGCAGACGCCGCAGATTCGATAACGTCAGGGTAGATGGTGCCCTGCGCCAGCCACTTCACGTCTTCCAGTTTCAGCGCTTCTTCATCGAATACTTCAACGAAAACGCGACCGATGATTTTACGTTTTGCTTCCGGATCGTTTTCGCCAGCCAGCGCTGACAGGAAG > NC_000913/2631395‑2631657
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
