Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I2 R1	77	34.8	1608548	95.0%	1528120	114.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,066,288	T→C	T296A (ACG→GCG)	ygfI ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,066,288	0	T	C	100.0%	144.3 / NA	39	T296A (ACG→GCG)	ygfI	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/19); total (20/19)

TCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAT  >  NC_000913/3066163‑3066392

tCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCt                                                                                             >  1:181786/1‑139 (MQ=255)

  gggATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTc                                                                                           >  1:149557/1‑139 (MQ=255)

    gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   >  1:152180/1‑129 (MQ=255)

    gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   <  2:152180/129‑1 (MQ=255)

    gATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAg                                                                                         <  2:159198/139‑1 (MQ=255)

      ttATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCa                                                                                       >  1:16967/1‑139 (MQ=255)

        aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAat                                                                                     >  2:191431/1‑139 (MQ=255)

        aTCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAat                                                                                     >  1:564289/1‑139 (MQ=255)

         tCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAata                                                                                    >  2:614085/1‑139 (MQ=255)

                   tATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCa                                                                          >  2:728974/1‑139 (MQ=255)

                          aaaaTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGc                                                                   >  2:287235/1‑139 (MQ=255)

                             aTGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGt                                                                <  1:359133/139‑1 (MQ=255)

                               gcgcCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGtat                                                              >  1:721245/1‑139 (MQ=255)

                                    tGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACga                                                         <  2:53478/139‑1 (MQ=255)

                                               ttCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGAt                                              <  1:724923/139‑1 (MQ=255)

                                                    cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         <  2:716922/139‑1 (MQ=255)

                                                    cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         <  2:378368/139‑1 (MQ=255)

                                                    cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         >  1:14471/1‑139 (MQ=255)

                                                    cacGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAAt                                         <  1:191431/139‑1 (MQ=255)

                                                        aTCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAg                                     >  1:537951/1‑139 (MQ=255)

                                                         tCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGa                                    <  2:14471/139‑1 (MQ=255)

                                                           ttAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  <  2:564289/139‑1 (MQ=255)

                                                           ttAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  <  2:721245/139‑1 (MQ=255)

                                                             aGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATAtt                                <  1:287235/139‑1 (MQ=255)

                                                              gCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTc                               >  1:90668/1‑139 (MQ=255)

                                                                   gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                          >  2:278370/1‑139 (MQ=255)

                                                                   gTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCa                          >  1:292200/1‑139 (MQ=255)

                                                                              acacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGc               <  1:82399/139‑1 (MQ=255)

                                                                               cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    >  2:356181/1‑85 (MQ=255)

                                                                               cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    <  1:356181/85‑1 (MQ=255)

                                                                               cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  >  2:213041/1‑119 (MQ=255)

                                                                               cacCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGcc              >  1:419103/1‑139 (MQ=255)

                                                                               cacCGTAAATTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGata                                  <  1:213041/119‑1 (MQ=255)

                                                                                  cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   >  2:197722/1‑51 (MQ=255)

                                                                                  cGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCa                                                                                                   <  1:197722/51‑1 (MQ=255)

                                                                                           attGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAt  <  2:645347/139‑1 (MQ=255)

                                                                                           attGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAt  <  2:90668/139‑1 (MQ=255)

                                                                                            ttGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    <  2:294603/72‑1 (MQ=255)

                                                                                            ttGCAACCGGCTCGATGCTGGGTTAATGACTCGCCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTgg                                                                    >  1:294603/1‑72 (MQ=255)

TCGGGATTATCTGCATCGCTATCTGGAAAATGCGCCTGGCGGACATATTCACCACGATCTTAGCCACGTCTTCGACTTACACCGTAATTTAATTGCAACCGGCTCGATGCTGGGTTAATGACTCGTCCCGTCACGATCTTCAGCAATATGCTGAAGCATCGTGGCGGTATAACGATGATTTCTGATTGAATAAAGATATTCCTGCATATACATCGGCCTGTGCGGTGCAT  >  NC_000913/3066163‑3066392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A