Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I2 R1
|
77 |
34.8 |
1608548 |
95.0% |
1528120 |
114.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,304,888 |
A→G |
N570N (AAT→AAC) |
yjcS ← |
metallo‑beta‑lactamase superfamily protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,304,888 | 0 | A | G | 96.0%
| 86.5
/ ‑4.0
| 25 | N570N (AAT→AAC) | yjcS | metallo‑beta‑lactamase superfamily protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/9); total (16/9) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 |
AGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAG > NC_000913/4304760‑4305019
|
aGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCg > 2:434867/1‑139 (MQ=255)
agGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAAc > 1:47315/1‑139 (MQ=255)
agGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAAc < 1:605584/139‑1 (MQ=255)
cGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCtt < 2:752090/117‑1 (MQ=255)
cGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCtt > 1:752090/1‑117 (MQ=255)
aGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCa < 1:271219/139‑1 (MQ=255)
ttCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCa > 2:714932/1‑118 (MQ=255)
ttCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCa < 1:714932/118‑1 (MQ=255)
cACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGa > 1:159494/1‑139 (MQ=255)
tATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCATCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGgt > 1:459918/1‑139 (MQ=255)
tATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGgt > 1:66725/1‑139 (MQ=255)
ttCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCg > 1:485377/1‑139 (MQ=255)
tCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCgg < 2:47315/139‑1 (MQ=255)
aGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCgggg > 2:142357/1‑139 (MQ=255)
aGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCgggg > 2:610174/1‑139 (MQ=255)
tCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAAcc > 2:328166/1‑139 (MQ=255)
aGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGc > 1:674639/1‑139 (MQ=255)
tCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGc < 2:159494/139‑1 (MQ=255)
gCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTg < 1:328166/139‑1 (MQ=255)
gTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAAc < 1:434867/139‑1 (MQ=255)
aGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTc > 1:539270/1‑139 (MQ=255)
gTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGCCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTcg > 2:757012/1‑139 (MQ=255)
tCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTcgcg > 2:176351/1‑139 (MQ=255)
aGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCa > 1:248679/1‑139 (MQ=255)
ggCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAg < 1:176351/139‑1 (MQ=255)
|
AGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAG > NC_000913/4304760‑4305019
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
