Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F1 I1 R1	81	47.9	2225048	94.8%	2109345	113.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,304,888	A→G	N570N (AAT→AAC)	yjcS ←	metallo‑beta‑lactamase superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,304,888	0	A	G	97.4%	146.0 / NA	39	N570N (AAT→AAC)	yjcS	metallo‑beta‑lactamase superfamily protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (23/15); minor base C (1/0); total (24/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGC  >  NC_000913/4304755‑4305020

cGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGttttt                                                                                                                                 >  1:569684/1‑139 (MQ=255)

                 cTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCgcgc                                                                                                                <  1:1084099/139‑1 (MQ=255)

                           aaGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCg                                                                                                      >  2:377163/1‑139 (MQ=255)

                              agGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCtt                                                                                                                      >  2:919169/1‑120 (MQ=255)

                              agGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCtt                                                                                                                      <  1:919169/120‑1 (MQ=255)

                              agGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAAc                                                                                                   >  2:559051/1‑139 (MQ=255)

                              agGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGATGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAAc                                                                                                   <  1:796572/139‑1 (MQ=255)

                               ggCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACg                                                                                                  >  2:248512/1‑139 (MQ=255)

                                gCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACgg                                                                                                 >  2:147267/1‑139 (MQ=255)

                                gCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACgg                                                                                                 >  2:851736/1‑139 (MQ=255)

                                 cGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGc                                                                                                >  2:203977/1‑139 (MQ=255)

                                 cGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGc                                                                                                >  1:992753/1‑139 (MQ=255)

                                   tCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCa                                                                                              >  1:446345/1‑139 (MQ=255)

                                     ggCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGc                                                                                                                           >  1:287256/1‑108 (MQ=255)

                                     ggCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGc                                                                                                                           <  2:287256/108‑1 (MQ=255)

                                          gCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTc                                                                                       <  2:992753/139‑1 (MQ=255)

                                              ttGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAAc                                                                                   <  1:377163/139‑1 (MQ=255)

                                              ttGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAAc                                                                                   <  2:326200/139‑1 (MQ=255)

                                                gCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAg                                                                                 <  1:203977/139‑1 (MQ=255)

                                                                        cGCTATCATTCAGCGTCAGGTTGAGAATATCGCCGTTGCTCATAATGAAGTTCAGGCTGATCGTTTTACCCGCAGATTTCGCGCTATCGAGGCGAACCGCCATAAAGTCTAACAGCATTTCGACCGACATCAcgagtat                                                         >  1:716403/1‑134 (MQ=255)

                                                                         gCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATg                                                        <  2:27973/139‑1 (MQ=255)

                                                                           tATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAg                                                                                         >  1:1021477/1‑104 (MQ=255)

                                                                           tATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAg                                                                                         <  2:1021477/104‑1 (MQ=255)

                                                                               gTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTcc                                                  >  2:324349/1‑139 (MQ=255)

                                                                                      gTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAAc                                           <  2:446345/139‑1 (MQ=255)

                                                                                       tCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAAcc                                          >  2:758385/1‑139 (MQ=255)

                                                                                               aGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGc                                  <  1:559051/139‑1 (MQ=255)

                                                                                                      cGCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCt                           >  2:1070698/1‑139 (MQ=255)

                                                                                                       gCCGTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTg                          <  1:248512/139‑1 (MQ=255)

                                                                                                          gTTGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAAc                       <  1:988451/139‑1 (MQ=255)

                                                                                                           ttGCTCATATTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACt                      >  1:442801/1‑139 (MQ=255)

                                                                                                                 atatTGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCa                <  1:1070698/139‑1 (MQ=255)

                                                                                                                     tGAAGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCAcccc            >  2:31209/1‑139 (MQ=255)

                                                                                                                        aGTTCAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTc         >  1:204684/1‑139 (MQ=255)

                                                                                                                            cAGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCgcgc     >  2:320771/1‑139 (MQ=255)

                                                                                                                             aGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAAGAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCAGAACTTATGTACCCCTTCGCGCa    >  1:732407/1‑139 (MQ=255)

                                                                                                                             aGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCa    >  1:919153/1‑139 (MQ=255)

                                                                                                                             aGGCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCa    >  1:184243/1‑139 (MQ=255)

                                                                                                                               gCTGATGTTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGc  >  1:354330/1‑139 (MQ=255)

CGTGCAGATCTTCACGGCTGATGTAGAAAGAGGCGTCGGCTTGCGGTTGCAGCGTTTTCCGGTAGTTAAGCACGCTATCGTTCAGCGTCAGGTTGAGGTTATCGCCGTTGCTCATATTGAAGTTCAGGCTGATATTTTTACCCGCAGCTTTCGCGCTATCGAGGCGAACGGCCATAAAGTCGAACAGCATTTCGACCGACATCCCGCGAATGGTGTCCGGGGAACCGGTGGTGCCGTGGCTGAACTTATGCACCCCTTCGCGCAGC  >  NC_000913/4304755‑4305020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A