Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
66 |
20.9 |
912876 |
97.7% |
891879 |
114.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,960,862 |
T→C |
T523A (ACC→GCC) |
recC ← |
exonuclease V (RecBCD complex), gamma chain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,960,862 | 0 | T | C | 100.0%
| 95.2
/ NA
| 26 | T523A (ACC→GCC) | recC | exonuclease V (RecBCD complex), gamma chain |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/11); total (15/11) |
GAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGC > NC_000913/2960732‑2960991
|
gAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTc > 1:303337/1‑139 (MQ=255)
aaGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTcc > 1:121343/1‑139 (MQ=255)
gATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCAt < 1:168014/139‑1 (MQ=255)
ggTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTc < 1:157373/88‑1 (MQ=255)
ggTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTc > 2:157373/1‑88 (MQ=255)
ggTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAAc > 1:159684/1‑139 (MQ=255)
ggTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAAc > 1:349485/1‑139 (MQ=255)
aaCCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACg < 2:424883/112‑1 (MQ=255)
aaCCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACg > 1:424883/1‑112 (MQ=255)
aTTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGa > 2:395557/1‑139 (MQ=255)
ttGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGAt > 2:221150/1‑139 (MQ=255)
ccTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCa < 2:349485/139‑1 (MQ=255)
ccTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCa < 1:66388/139‑1 (MQ=255)
ccTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCa < 2:159684/139‑1 (MQ=255)
gcgcTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGc < 2:303337/139‑1 (MQ=255)
ctcCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTaaa > 1:27750/1‑139 (MQ=255)
gTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGc > 2:88370/1‑125 (MQ=255)
gTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGc < 1:88370/125‑1 (MQ=255)
aGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATg < 1:43542/101‑1 (MQ=255)
aGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATg > 2:43542/1‑101 (MQ=255)
aCGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCAt > 2:194797/1‑71 (MQ=255)
aCGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCAt < 1:194797/71‑1 (MQ=255)
aGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGc > 2:357192/1‑103 (MQ=255)
aGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGc < 1:357192/103‑1 (MQ=255)
aTCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGcgccgc > 2:191181/1‑139 (MQ=255)
aTCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGcgccgc > 1:44094/1‑139 (MQ=255)
|
GAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGC > NC_000913/2960732‑2960991
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
