Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I2 R1
|
65 |
21.2 |
1002576 |
96.5% |
967485 |
111.4 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,960,862 |
T→C |
T523A (ACC→GCC) |
recC ← |
exonuclease V (RecBCD complex), gamma chain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,960,862 | 0 | T | C | 100.0%
| 93.6
/ NA
| 25 | T523A (ACC→GCC) | recC | exonuclease V (RecBCD complex), gamma chain |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/11); total (14/11) |
GAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCA > NC_000913/2960732‑2960996
|
gAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTc > 1:303140/1‑139 (MQ=255)
cAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTg < 2:381512/139‑1 (MQ=255)
gATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGc > 1:380462/1‑139 (MQ=255)
aTGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCg > 1:277941/1‑139 (MQ=255)
tAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACg < 2:380462/139‑1 (MQ=255)
gCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACg > 2:44166/1‑133 (MQ=255)
gCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACg < 1:44166/133‑1 (MQ=255)
gCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTgtcg < 2:277941/139‑1 (MQ=255)
ggTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAAc > 2:464937/1‑139 (MQ=255)
aGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGaa > 2:387163/1‑139 (MQ=255)
cGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCg > 1:55163/1‑139 (MQ=255)
ttGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAAc > 1:421460/1‑105 (MQ=255)
ttGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAAc < 2:421460/105‑1 (MQ=255)
cTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTg < 1:464937/139‑1 (MQ=255)
ccTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCa < 1:387163/139‑1 (MQ=255)
ctctCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTa < 2:55163/139‑1 (MQ=255)
gTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGc > 1:340753/1‑139 (MQ=255)
tACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCggg < 2:78265/43‑1 (MQ=255)
tACGCGTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCggg > 1:78265/1‑43 (MQ=255)
gTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTa < 2:100591/109‑1 (MQ=255)
gTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTa > 1:100591/1‑109 (MQ=255)
gTCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCaa > 2:449352/1‑139 (MQ=255)
tCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGTGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAg < 1:304200/118‑1 (MQ=255)
tCAGGCCAAATCGCCAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCGCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAg > 2:304200/1‑118 (MQ=255)
cAGGCGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCa > 1:213078/1‑139 (MQ=255)
|
GAAGCCAGATGCCCCACCAGTTCTGCAATTAAGCCGCTCGATTCATCATAAGGTAGAACCGATTGCCACTCGCCCTGCGCGCTCTCCATCGCGTAGCCCAACAACATACGCGTCAGGCCAAATCGCCAGGTGTGTTGTCCGGTGGCGGGGAGTTCCAGCTCGCGAACGTTGTCGTCATCTATGCCCCAACGAATGCCGGATTCGTTGACCCACTGGCGTAAATAACGCAGCCCTTCTTCGGTGATGTCAAACCGCGCCGCCAGCA > NC_000913/2960732‑2960996
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
