Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F1 I1 R1 65 25.3 1027584 98.0% 1007032 120.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,040,142 T→C V222A (GTA→GCA)  yedY → membrane‑anchored, periplasmic TMAO, DMSO reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,040,1420TC100.0% 50.3 / NA 15V222A (GTA→GCA) yedYmembrane‑anchored, periplasmic TMAO, DMSO reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/9);  total (6/9)

GTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGT  >  NC_000913/2040006‑2040238
                                                                                                                                        |                                                                                                
gTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTTATGACCGCAg                                                                                                <  1:102177/139‑1 (MQ=255)
                         cGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGcc                                                                       <  1:339942/139‑1 (MQ=255)
                         cGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGcc                                                                       >  2:339942/1‑139 (MQ=255)
                                      aggccagCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGgcgcg                                                          >  2:10036/1‑139 (MQ=255)
                                           agcagGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGt                                                     >  2:391810/1‑139 (MQ=255)
                                                           cggcggTGGGCTGAAATATCCTCATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGATTGATTGTGCCg                                     <  2:149190/139‑1 (MQ=255)
                                                                   ggCTGAATTATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAata                             <  2:36364/139‑1 (MQ=255)
                                                                     cTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATg                           <  2:289135/139‑1 (MQ=255)
                                                                            atCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATACATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTaa                    <  2:333268/139‑1 (MQ=255)
                                                                                        aaGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATc        >  2:201255/1‑139 (MQ=255)
                                                                                         aGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg       <  1:175668/139‑1 (MQ=255)
                                                                                         aGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCg       >  2:20149/1‑139 (MQ=255)
                                                                                          ggATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGa      <  2:196973/139‑1 (MQ=255)
                                                                                              tGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt  <  1:10036/139‑1 (MQ=255)
                                                                                              tGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGCAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGt  >  1:111092/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                
GTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTGTTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAAAGGGATTAAATCGATAGT  >  NC_000913/2040006‑2040238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A