Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F1 I2 R1	69	53.0	2252012	96.1%	2164183	115.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	630,819	C→T	A309V (GCG→GTG)	cstA →	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	630,819	0	C	T	100.0%	109.2 / NA	32	A309V (GCG→GTG)	cstA	carbon starvation protein involved in peptide utilization, APC peptide transporter family protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (17/15); total (17/15)

GCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAC  >  NC_000913/630695‑630939

gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAAttt                                                                                                             >  2:757283/1‑138 (MQ=255)

gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAAttt                                                                                                             <  1:757283/138‑1 (MQ=255)

    tgctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGa                                                                                                        <  2:514034/139‑1 (MQ=255)

              gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   <  1:233741/134‑1 (MQ=255)

              gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   >  2:233741/1‑134 (MQ=255)

              gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGc                                                                                              <  1:1072479/139‑1 (MQ=255)

                ggTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGccc                                                                                            >  1:1087196/1‑139 (MQ=255)

                      tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATg                                                                                      <  2:66373/139‑1 (MQ=255)

                      tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATg                                                                                      >  1:66373/1‑139 (MQ=255)

                           gCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCg                                                                                 >  2:337045/1‑139 (MQ=255)

                                gcgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAAc                                                                            <  2:1087196/139‑1 (MQ=255)

                                          ctctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGt                                                                  <  2:162693/139‑1 (MQ=255)

                                             tctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTcc                                                               >  1:777758/1‑139 (MQ=255)

                                             tctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTcc                                                               <  1:291231/139‑1 (MQ=255)

                                                   ttCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTa                                                         <  1:337045/139‑1 (MQ=255)

                                                    tCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                        >  1:611563/1‑139 (MQ=255)

                                                    tCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                        >  2:287452/1‑139 (MQ=255)

                                                     ccTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATc                                                       >  1:471717/1‑139 (MQ=255)

                                                                ggACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                            >  1:861321/1‑139 (MQ=255)

                                                                     aTCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgcg                                       <  1:287452/139‑1 (MQ=255)

                                                                           ggTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCa                                                                                                                  >  2:161744/1‑58 (MQ=255)

                                                                           ggTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCa                                                                                                                  <  1:161744/58‑1 (MQ=255)

                                                                                ggCGGTAGGCATTTTGATTATGCGCCCGACGCTGACTATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGAAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                            >  1:714344/1‑139 (MQ=255)

                                                                                 gCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   <  2:19304/67‑1 (MQ=255)

                                                                                 gCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCa                                                                                                   >  1:19304/1‑67 (MQ=255)

                                                                                           ttttGATTATGCGCCCGACGCTGACCATGCCTGTGCTGAc                                                                                                                    <  1:473224/40‑1 (MQ=38)

                                                                                           ttttGATTATGCGCCCGACGCTGACCATGCCTGTGCTGAc                                                                                                                    >  2:473224/1‑40 (MQ=38)

                                                                                                aTTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtct            >  1:194879/1‑139 (MQ=255)

                                                                                                aTTATGCGCCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtct            <  2:777758/139‑1 (MQ=255)

                                                                                                      cgcCCGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCATTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTg      >  2:711121/1‑139 (MQ=255)

                                                                                                          cGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGTCCGGGATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc  <  1:843960/139‑1 (MQ=255)

                                                                                                          cGACGCTGACCATGCCTGTGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc  >  2:935698/1‑139 (MQ=255)

GCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGACCATGCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAC  >  NC_000913/630695‑630939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A