Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A12 F1 I2 R1
|
69 |
53.0 |
2252012 |
96.1% |
2164183 |
115.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,629,489 |
G→A |
G475G (GGC→GGT) |
ydfI ← |
putative NAD‑dependent D‑mannonate oxidoreductase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,629,489 | 0 | G | A | 98.4%
| 219.7
/ NA
| 63 | G475G (GGC→GGT) | ydfI | putative NAD‑dependent D‑mannonate oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (28/34); minor base T (0/1); total (28/35) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 |
TGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTA > NC_000913/1629358‑1629618
|
tgttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg < 2:40855/139‑1 (MQ=255)
tgttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg < 2:1063933/139‑1 (MQ=255)
gttgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcgc > 1:717927/1‑139 (MQ=255)
tgAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCta < 2:503723/139‑1 (MQ=255)
gTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATaaa > 2:13126/1‑139 (MQ=255)
gtgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGAc > 2:874268/1‑139 (MQ=255)
tgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg > 1:216750/1‑127 (MQ=255)
tgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg < 2:216750/127‑1 (MQ=255)
tgAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACa > 2:942202/1‑139 (MQ=255)
aaaCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGt > 1:1053144/1‑139 (MQ=255)
aaaCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGt > 2:467151/1‑139 (MQ=255)
aaaCTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGCCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGt < 2:804008/139‑1 (MQ=255)
gACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGc < 1:273630/139‑1 (MQ=255)
cTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGt < 2:438473/139‑1 (MQ=255)
aTTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg > 1:357029/1‑99 (MQ=255)
aTTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg < 2:357029/99‑1 (MQ=255)
tctcGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaac < 1:481444/139‑1 (MQ=255)
tctcGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTaaacaaac > 1:292564/1‑139 (MQ=255)
cATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGt < 1:1068626/139‑1 (MQ=255)
tGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCt < 1:959414/139‑1 (MQ=255)
aaCTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAAT‑AAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGc > 1:839338/1‑139 (MQ=255)
tAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCaa < 2:688452/139‑1 (MQ=255)
aaGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAAT‑AAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAat < 2:839338/139‑1 (MQ=255)
aGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAat < 2:717927/139‑1 (MQ=255)
cGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCGGTAAACAAACTATTGTCTGGCAAatca < 2:19866/139‑1 (MQ=255)
cGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatca < 2:27861/139‑1 (MQ=255)
gtgtGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatcat < 1:467151/139‑1 (MQ=255)
tgtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatcatc > 2:826554/1‑139 (MQ=255)
gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg > 1:408738/1‑74 (MQ=255)
gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATgcg < 2:408738/74‑1 (MQ=255)
gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAAtcatca > 1:80136/1‑139 (MQ=255)
gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAAtcatca > 1:356520/1‑139 (MQ=255)
gtgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAAtcatca > 1:470801/1‑139 (MQ=255)
tgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCacg < 2:326511/71‑3 (MQ=255)
tgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCacg > 1:326511/1‑69 (MQ=255)
tgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACa < 2:1007591/85‑1 (MQ=255)
tgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACa > 1:1007591/1‑85 (MQ=255)
tgAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCAc < 1:13126/139‑1 (MQ=255)
cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCTCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatc < 1:697934/127‑1 (MQ=255)
cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAatc > 2:697934/1‑127 (MQ=255)
cgccgATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGAt > 2:903632/1‑139 (MQ=255)
ggcggcATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCAGGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTa < 1:774115/139‑1 (MQ=255)
gcATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCg > 1:577948/1‑139 (MQ=255)
gcATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCg < 2:577948/139‑1 (MQ=255)
tGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAg > 2:82431/1‑139 (MQ=255)
tGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAg < 2:536269/139‑1 (MQ=255)
aCTTCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTg < 2:78648/139‑1 (MQ=255)
tCACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGAc > 2:630325/1‑139 (MQ=255)
cACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCa > 2:974797/1‑65 (MQ=255)
cACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCa < 1:974797/65‑1 (MQ=255)
cACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGagg > 1:7475/1‑137 (MQ=255)
cACGGAATATTTTGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGAcg < 2:167455/139‑1 (MQ=255)
tGCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCt > 1:45261/1‑139 (MQ=255)
gCCACGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCtt < 1:82431/139‑1 (MQ=255)
cGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGt < 2:656870/46‑1 (MQ=255)
cGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGt > 1:656870/1‑46 (MQ=255)
cGGTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGc > 2:570526/1‑139 (MQ=255)
ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa < 2:45261/139‑1 (MQ=255)
ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa < 2:292564/139‑1 (MQ=255)
ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa < 1:903632/139‑1 (MQ=255)
ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa > 1:599135/1‑139 (MQ=255)
ggTCGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCa < 1:570526/139‑1 (MQ=255)
cGCTTTCGCACCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCacta < 1:942202/139‑1 (MQ=255)
|
TGTTGAAGGTTGTGAAGAGAAACTGGCACCACTGGACCTGATTCGTCTCGGCATGAACTAAGCGTGTGAATGCCGCCGATGGCGGCATTGCTTTTTTACTTCACGGAATATTTTGCCACGGTCGCTTTCGCGCCATGCGCTAATAAAGACAAGTACGTTTCCGTCACTCTTGCAGTAAACAAACTATTGTCTGGCAAATCATCACCAAAGATCGCCTTAATCGCCAGCAATGACTGGACGCGCGCTTTCCCTTCGGCACTA > NC_000913/1629358‑1629618
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
